Living fabrication of functional semi-interpenetrating polymeric materials.
Living fabrication of functional semi-interpenetrating polymeric materials.
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DOI：
10.1038/s41467-021-23812-7
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发表时间：
2021-06-08
影响因子：
16.6
通讯作者：
You L
中科院分区：
文献类型：
--
作者：
Dai Z;Yang X;Wu F;Wang L;Xiang K;Li P;Lv Q;Tang J;Dohlman A;Dai L;Shen X;You L
Cell-mediated living fabrication has great promise for generating materials with versatile, programmable functions. Here, we demonstrate the engineering of living materials consisting of semi-interpenetrating polymer networks (sIPN). The fabrication process is driven by the engineered bacteria encapsulated in a polymeric microcapsule, which serves as the initial scaffold. The bacteria grow and undergo programmed lysis in a density-dependent manner, releasing protein monomers decorated with reactive tags. Those protein monomers polymerize with each other to form the second polymeric component that is interlaced with the initial crosslinked polymeric scaffold. The formation of sIPN serves the dual purposes of enhancing the mechanical property of the living materials and anchoring effector proteins for diverse applications. The material is resilient to perturbations because of the continual assembly of the protein mesh from the monomers released by the engineered bacteria. We demonstrate the adoption of the platform to protect gut microbiota in animals from antibiotic-mediated perturbations. Our work lays the foundation for programming functional living materials for diverse applications. Cell based materials production has potential for generating diverse materials with a range of functions. Here, the authors report development of living fabrication of biohybrid semi interpenetrating polymer networks by encapsulating protein producing bacteria within polymer microcapsules.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
21.8
作者：
Krall, Nikolaus;da Cruz, Filipa P.;Bernardes, Goncalo J. L.
通讯作者：
Bernardes, Goncalo J. L.
影响因子：
17.1
作者：
Gu, Zhen;Dang, Tram T.;Anderson, Daniel G.
通讯作者：
Anderson, Daniel G.
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DOI：
10.1126/science.aas9315
发表时间：
2018-05-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Mimee M;Nadeau P;Hayward A;Carim S;Flanagan S;Jerger L;Collins J;McDonnell S;Swartwout R;Citorik RJ;Bulović V;Langer R;Traverso G;Chandrakasan AP;Lu TK
通讯作者：
Lu TK
影响因子：
2.8
作者：
Datta, Sumitra;Christena, L. Rene;Rajaram, Yamuna Rani Sriramulu
通讯作者：
Rajaram, Yamuna Rani Sriramulu