Soft X-ray tomography and cryogenic light microscopy: the cool combination in cellular imaging.
Soft X-ray tomography and cryogenic light microscopy: the cool combination in cellular imaging.
复制标题
DOI：
10.1016/j.tcb.2009.08.005
复制
发表时间：
2009-11
影响因子：
19
通讯作者：
Larabell, Carolyn A.
中科院分区：
文献类型：
--
作者：
McDermott, Gerry;Le Gros, Mark A.;Knoechel, Christian G.;Uchida, Maho;Larabell, Carolyn A.
软X射线断层扫描（SXT）非常适合亚细胞结构和组织成像，特别是在真核细胞中。SXT在概念上类似于成熟的医学诊断技术计算机轴向断层扫描（CAT），除了SXT能够以50 nm或更高的空间分辨率成像。在软X射线断层扫描（SXT）中，细胞使用来自被称为“水窗”的光谱区域的光子成像。这导致完整的、完全水合的细胞的定量、高对比度图像，而不需要使用对比度增强剂。因此，细胞的可视化非常接近其天然的、功能齐全的状态。SXT的实用性最近得到了增强的相关成像的高数值孔径低温光学显微镜的发展。采用这种多模态方法现在允许标记的分子在细胞的高分辨率三维断层重建的背景下被定位。
Soft x-ray tomography (SXT) is ideally suited to imaging sub-cellular architecture and organization, particularly in eukaryotic cells. SXT is similar in concept to the well-established medical diagnostic technique computed axial tomography (CAT), except SXT is capable of imaging with a spatial resolution of 50 nm, or better. In soft x-ray tomography (SXT) cells are imaged using photons from a region of the spectrum known as the ‘water window’. This results in quantitative, high-contrast images of intact, fully hydrated cells without the need to use contrast-enhancing agents. Cells are therefore visualized very close to their native, fully functional state. The utility of SXT has recently been enhanced by the development of high numerical aperture cryogenic light microscopy for correlated imaging. Taking this multi-modal approach now allows labeled molecules to be localized in the context of a high-resolution 3-dimensional tomographic reconstruction of the cell.
登录
查看更多内容
影响因子：
2.9
作者：
Gu, Weiwei;Etkin, Laurence D.;Larabell, Carolyn A.
通讯作者：
Larabell, Carolyn A.
影响因子：
3
作者：
Hildebrand, Mark;Kim, Sang;Shi, Dan;Scott, Keana;Subramaniam, Sriram
通讯作者：
Subramaniam, Sriram
影响因子：
3.3
作者：
Larabell, CA;Le Gros, MA
通讯作者：
Le Gros, MA
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3
作者：
Hohn, Michael;Tang, Grant;Ludtke, Steven J.
通讯作者：
Ludtke, Steven J.