3D imaging of diatoms with ion-abrasion scanning electron microscopy.
3D imaging of diatoms with ion-abrasion scanning electron microscopy.
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DOI：
10.1016/j.jsb.2009.02.014
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发表时间：
2009-06
影响因子：
3
通讯作者：
Subramaniam, Sriram
中科院分区：
文献类型：
--
作者：
Hildebrand, Mark;Kim, Sang;Shi, Dan;Scott, Keana;Subramaniam, Sriram
离子磨损扫描电子显微镜(IASEM)利用聚焦的离子束对块状样品表面的薄片进行研磨，并结合扫描电子显微镜对每个切片的表面进行成像，从而能够以~30 nm的分辨率重建亚细胞结构。在这里，我们报告了第一次应用IASEM对生物矿化有机体--海洋硅藻--假海链藻进行成像。硅藻具有高度图案化的硅基细胞壁结构，是生物系统定向合成纳米材料研究和应用的独特模型。我们的研究为电池的“硬”(硅质)和“软”(有机)组件的结构和组装原理提供了新的见解。通过在不同阶段捕获的发育同步硅藻的3D重建，我们表明微米和纳米级的硅质结构都可以在它们形成的特定阶段被可视化。我们不仅证明了结构在整个细胞环境中是可视化的，而且还证明了脆弱的早期结构是可见的，并且这可以与暴露切片中的元素映射相结合。我们展示了二氧化硅结构的3D结构，以及调节其创建和定位的细胞组件可以同时可视化，为在遗传易处理的系统中研究和操纵矿物纳米结构提供了新的机会。
Ion-abrasion scanning electron microscopy (IASEM) takes advantage of focused ion beams to abrade thin sections from the surface of bulk specimens, coupled with SEM to image the surface of each section, enabling 3D reconstructions of subcellular architecture at ~ 30 nm resolution. Here, we report the first application of IASEM for imaging a biomineralizing organism, the marine diatom Thalassiosira pseudonana. Diatoms have highly patterned silica-based cell wall structures that are unique models for the study and application of directed nanomaterials synthesis by biological systems. Our study provides new insights into the architecture and assembly principles of both the “hard” (siliceous) and “soft” (organic) components of the cell. From 3D reconstructions of developmentally synchronized diatoms captured at different stages, we show that both micro- and nanoscale siliceous structures can be visualized at specific stages in their formation. We show that not only are structures visualized in a whole-cell context, but demonstrate that fragile, early-stage structures are visible, and that this can be combined with elemental mapping in the exposed slice. We demonstrate that the 3D architectures of silica structures, and the cellular components that mediate their creation and positioning can be visualized simultaneously, providing new opportunities to study and manipulate mineral nanostructures in a genetically tractable system.
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影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
7
作者：
Frigeri, LG;Radabaugh, TR;Hildebrand, M
通讯作者：
Hildebrand, M
影响因子：
2.7
作者：
Hildebrand, Mark;York, Evelyn;Doktycz, Mitchel J.
通讯作者：
Doktycz, Mitchel J.
影响因子：
64.8
作者：
Bao, Zhihao;Weatherspoon, Michael R.;Sandhage, Kenneth H.
通讯作者：
Sandhage, Kenneth H.
影响因子：
--
作者：
Hildebrand, M
通讯作者：
Hildebrand, M