Development of biodegradable nanocarriers loaded with a monoclonal antibody.
Development of biodegradable nanocarriers loaded with a monoclonal antibody.
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DOI：
10.3390/ijms16023990
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发表时间：
2015-02-12
影响因子：
5.6
通讯作者：
Vishwanatha J
中科院分区：
文献类型：
--
作者：
Gdowski A;Ranjan A;Mukerjee A;Vishwanatha J
利用单克隆抗体治疗剂针对癌细胞中的细胞内蛋白质-蛋白质相互作用的治疗受到几个因素的阻碍，包括细胞内摄取差和快速溶酶体降解。我们目前的工作探讨了使用水/油/水双乳液溶剂蒸发技术将单克隆抗体包封在聚（乳酸-羟基乙酸）（PLGA）纳米颗粒内的可行性。该方法可用于制备保护性聚合物纳米颗粒，用于将功能性抗体转运至癌细胞的胞质区室。纳米颗粒的配制，然后使用许多物理和生物参数进行表征。平均纳米颗粒尺寸范围为221至252 nm，具有低的多分散指数。包封率为16%-22%，抗体载量为0.3%-1.12%。抗体分子以持续的方式从纳米颗粒释放，并且在释放时保持功能性。我们的研究成功配制了负载抗体的聚合物纳米颗粒，从而表明基于PLGA的抗体纳米制剂是癌细胞中大量新细胞内抗体靶点的有前途的细胞内递送载体。
Treatments utilizing monoclonal antibody therapeutics against intracellular protein-protein interactions in cancer cells have been hampered by several factors, including poor intracellular uptake and rapid lysosomal degradation. Our current work examines the feasibility of encapsulating monoclonal antibodies within poly(lactic-co-glycolic acid) (PLGA) nanoparticles using a water/oil/water double emulsion solvent evaporation technique. This method can be used to prepare protective polymeric nanoparticles for transporting functional antibodies to the cytoplasmic compartment of cancer cells. Nanoparticles were formulated and then characterized using a number of physical and biological parameters. The average nanoparticle size ranged from 221 to 252 nm with a low polydispersity index. Encapsulation efficiency of 16%–22% and antibody loading of 0.3%–1.12% were observed. The antibody molecules were released from the nanoparticles in a sustained manner and upon release maintained functionality. Our studies achieved successful formulation of antibody loaded polymeric nanoparticles, thus indicating that a PLGA-based antibody nanoformulation is a promising intracellular delivery vehicle for a large number of new intracellular antibody targets in cancer cells.
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影响因子：
5.1
作者：
Lü JM;Wang X;Marin-Muller C;Wang H;Lin PH;Yao Q;Chen C
通讯作者：
Chen C
影响因子：
10.8
作者：
Panyam, J;Dali, MA;Labhasetwar, V
通讯作者：
Labhasetwar, V
影响因子：
--
作者：
Braden, Arthur R.;Kafka, Michael I.;Vishwanatha, Jamboor K.
通讯作者：
Vishwanatha, Jamboor K.
影响因子：
4.8
作者：
Panyam, J;Zhou, WZ;Labhasetwar, V
通讯作者：
Labhasetwar, V
DOI：
10.1073/pnas.0809154105
发表时间：
2008-11-11
影响因子：
11.1
作者：
Dhar, Shanta;Gu, Frank X.;Lippard, Stephen J.
通讯作者：
Lippard, Stephen J.