Acquisition of androgen-mediated expression of mouse vas deferens protein (MVDP) gene in cultured epithelial cells and in vas deferens during postnatal development.
Acquisition of androgen-mediated expression of mouse vas deferens protein (MVDP) gene in cultured epithelial cells and in vas deferens during postnatal development.
复制标题
在培养的上皮细胞和出生后发育期间的输精管中获得雄激素介导的小鼠输精管蛋白（MVDP）基因的表达。
作者：
M. Manin;Antoine Martinez;Bernadette van der SCHUEREN;Ingrid Reynaert;C. Jean
我们使用培养的输精管上皮细胞（VDEC）作为模型系统，以确定条件，允许小鼠输精管蛋白（MVDP）基因表达和收购雄激素的反应。北方印迹分析的基础上，mvdp基因的组成型表达在非常低的水平，在青春期前的VDEC上生长的胶原包被的塑料或微孔膜插入。在双氢睾酮（DHT）的存在下，mvdp信使RNA水平显着增加，在微孔膜插入物上培养的细胞，并保持不变，在基质涂层塑料上生长的细胞。来自胎儿输精管的上皮细胞能够合成MVDP响应DHT，胎儿间充质细胞的存在并不影响MVDP的生产。为细胞提供允许进入基底外侧膜并满足细胞极性要求的培养程序是雄激素诱导MVDP基因表达的先决条件。研究结果还指出表皮生长因子、胰岛素和酪氨酸激酶活性在介导雄激素对mvdp基因表达的作用。体内研究表明，产后5 - 7天mvdp基因的首次表达与上皮细胞的主要结构变化无关。上皮细胞和管周收缩细胞在10 - 20天获得成熟表型与mvdp基因的雄激素依赖性相关。我们提出，细胞分化和极化的基质涂层微孔膜再现一些事件，是必要的收购雄激素反应的mvdp基因在出生后的发展。
We used cultured vas deferens epithelial cells (VDECs) as a model system to determine the conditions that allow mouse vas deferens protein (MVDP) gene expression and acquisition of androgen responsiveness. On the basis of Northern blot analysis, the mvdp gene is constitutively expressed at very low levels in prepubertal VDECs grown on collagen-coated plastic or on microporous membrane inserts. In the presence of dihydrotestosterone (DHT), mvdp messenger RNA levels dramatically increased in cells cultured on microporous membrane inserts and stayed unchanged in cells grown on matrix-coated plastic. Epithelial cells derived from fetal vas deferens were able to synthesize MVDP in response to DHT, and the presence of fetal mesenchymal cells did not influence MVDP production. Providing the cells with a culture procedure that permits access to the basolateral membranes and caters to the polarity requirements of the cell is a prerequisite for androgen induction of MVDP gene expression. The results also point to a role for epidermal growth factor, insulin, and tyrosine kinase activity in mediating the action of androgen on mvdp gene expression. In vivo studies show that the first expression of the mvdp gene between 5 and 7 days postpartum is not associated with major structural changes in the epithelium. The acquisition of a mature phenotype by epithelial and peritubular contractile cells, between 10 and 20 days, correlates with androgen dependency of the mvdp gene. We propose that cell differentiation and polarization on a matrix-coated microporous membrane reproduces some of the events that are necessary for acquisition of androgenic responsiveness of the mvdp gene during postnatal development.
登录
查看更多内容
影响因子：
--
作者：
Gerald R. Cunha;Elaine T. Alarid;Timothy Turner;A. Donjacour;E. Boutin;B. A. Foster
通讯作者：
Gerald R. Cunha;Elaine T. Alarid;Timothy Turner;A. Donjacour;E. Boutin;B. A. Foster
胎鼠生殖道分化过程中表皮生长因子基因表达的刺激：雄激素及其受体的作用。
DOI：
10.1210/endo.137.2.8593821
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Gupta,C;Singh,M
通讯作者：
Singh,M
表皮生长因子受体（EGFR）在男性生殖道分化中的作用：用抗EGFR抗体刺激EGFR表达并抑制沃尔夫管分化。
DOI：
10.1210/endo.137.3.8603602
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Gupta,C
通讯作者：
Gupta,C
影响因子：
4.8
作者：
COOKE, PS;YOUNG, P;CUNHA, GR
通讯作者：
CUNHA, GR
影响因子：
4.8
作者：
CHAREST, NJ;PETRUSZ, P;FRENCH, FS
通讯作者：
FRENCH, FS