Nuclear RNP complex assembly initiates cytoplasmic RNA localization.
Nuclear RNP complex assembly initiates cytoplasmic RNA localization.
复制标题
核RNP复合物组装启动细胞质RNA定位。
DOI：
10.1083/jcb.200309145
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发表时间：
2004-04-26
期刊：
影响因子：
--
通讯作者：
Mowry KL
中科院分区：
文献类型：
--
作者：
Kress TL;Yoon YJ;Mowry KL
mRNA的细胞质定位是产生细胞极性的普遍机制，并且可以为胚胎发育期间的模式化提供基础。这方面的一个突出的例子是在非洲爪蟾卵母细胞中的母体mRNA的定位，这一过程需要通过定位机制的蛋白质组分识别必需的RNA序列。然而，目前尚不清楚这些蛋白质因子如何以及何时与定位的RNA结合以进行RNA转运。为了追踪介导RNA定位的RNA-蛋白质相互作用，我们分析了来自细胞核和细胞质的RNP复合物。我们发现，在本地化途径的早期步骤是识别本地化的RNA在细胞核中的特定RNA结合蛋白。转运到细胞质后，RNP复合物被重塑，并招募额外的转运因子。这些结果表明，细胞质RNA定位起始于细胞核中，并且细胞核中特异性RNA结合蛋白的结合可以将RNA靶向至细胞质中的适当目的地。
Cytoplasmic localization of mRNAs is a widespread mechanism for generating cell polarity and can provide the basis for patterning during embryonic development. A prominent example of this is localization of maternal mRNAs in Xenopus oocytes, a process requiring recognition of essential RNA sequences by protein components of the localization machinery. However, it is not yet clear how and when such protein factors associate with localized RNAs to carry out RNA transport. To trace the RNA–protein interactions that mediate RNA localization, we analyzed RNP complexes from the nucleus and cytoplasm. We find that an early step in the localization pathway is recognition of localized RNAs by specific RNA-binding proteins in the nucleus. After transport into the cytoplasm, the RNP complex is remodeled and additional transport factors are recruited. These results suggest that cytoplasmic RNA localization initiates in the nucleus and that binding of specific RNA-binding proteins in the nucleus may act to target RNAs to their appropriate destinations in the cytoplasm.
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