A standardized Humulus lupulus (L.) ethanol extract partially prevents ovariectomy-induced bone loss in the rat without induction of adverse effects in the uterus.
A standardized Humulus lupulus (L.) ethanol extract partially prevents ovariectomy-induced bone loss in the rat without induction of adverse effects in the uterus.
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DOI：
10.1016/j.phymed.2017.08.001
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发表时间：
2017-10-15
期刊：
影响因子：
--
通讯作者：
Zierau O
中科院分区：
文献类型：
--
作者：
Keiler AM;Helle J;Bader MI;Ehrhardt T;Nestler K;Kretzschmar G;Bernhardt R;Vollmer G;Nikolić D;Bolton JL;Pauli GF;Chen SN;Dietz BM;van Breemen RB;Zierau O
啤酒花（Humulus lupulus（L.））膳食补充剂作为减轻更年期症状如潮热、抑郁和焦虑的草药疗法是令人感兴趣的。到目前为止，关于啤酒花制剂的雌激素和相关特性的证据被认为不足以批准绝经适应症的市场。该研究旨在研究化学标准化的啤酒花提取物在子宫中的安全性，威尔斯其在卵巢切除大鼠模型中预防骨质流失的功效。雌性Wistar大鼠卵巢切除，并分为对照组，接受植物雌激素的饮食，一组与E2苯甲酸酯（0.93毫克/公斤体重/天）和一组与标准化啤酒花提取物（60毫克/公斤体重/天）治疗8周。分析胫骨和椎骨的微计算机断层扫描以及子宫和胫骨的组织学变化。口服啤酒花提取物8周后，在子宫内膜中未观察到子宫肥大或增殖作用。然而，观察到部位依赖性骨骼效应。啤酒花提取物显着减少胫骨干骺端破骨细胞的数量，并防止减少骨小梁厚度，导致雌二醇耗尽。相比之下，啤酒花提取物不能防止卵巢切除术引起的腰椎微结构变化。甚至发现某些参数（例如骨小梁的厚度和数量）低于卵巢切除对照组中测定的值。总之，结果为标准化啤酒花提取物的安全性提供了证据，并指出了雌二醇消耗后对骨丢失的弱骨类型特异性保护作用。
Hops (Humulus lupulus (L.)) dietary supplements are of interest as herbal remedies to alleviate menopausal symptoms, such as hot flushes, depression and anxiety. So far, the evidence regarding estrogenic and related properties of hops preparations has been considered insufficient for a market authorization for menopausal indications. The study aims to investigate a chemically standardized hops extract regarding its safety in the uterus, as wells as its efficacy to prevent bone loss in the ovariectomized rat model. Female Wistar rats were ovariectomized and divided into a control group receiving phytoestrogen-free diet, a group treated with E2benzoate (0.93 mg/kg body weight/d) and a group treated with the standardized hops extract (60 mg/kg body weight/d) for 8 weeks. Micro-computed tomography of the tibiae and vertebrae, as wells as histological changes in the uterus and tibia were analyzed. Neither uterotrophic nor proliferative effects were observed in the endometrium in response to the oral 8-week administration of the hops extract. However, site-dependent skeletal effects were observed. The hops extract significantly decreased the number of osteoclasts in the tibial metaphysis and prevented reduction of the trabecular thickness that resulted from estradiol depletion. In contrast, the hops extract did not prevent the ovariectomy-induced micro-architectural changes in the lumbar vertebra. Certain parameters (e.g. thickness and number of trabeculae) were even found to be below the values determined in the ovariectomized control group. Taken together, the results provide evidence for the safety of the standardized hops extract and point to a weak bone type-specific, protective effect on bone loss following estradiol depletion.
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影响因子：
1.6
作者：
Tobe, H;Muraki, Y;Nagai, M
通讯作者：
Nagai, M
DOI：
10.1016/j.jsbmb.2005.05.009
发表时间：
2005-11-01
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影响因子：
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通讯作者：
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