A novel human ex vivo skin model to study early local responses to burn injuries.
A novel human ex vivo skin model to study early local responses to burn injuries.
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DOI：
10.1038/s41598-020-79683-3
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发表时间：
2021-01-11
影响因子：
4.6
通讯作者：
Kotzbeck P
中科院分区：
文献类型：
--
作者：
Hofmann E;Fink J;Eberl A;Prugger EM;Kolb D;Luze H;Schwingenschuh S;Birngruber T;Magnes C;Mautner SI;Kamolz LP;Kotzbeck P
烧伤会引发许多过程，如热休克反应、炎症和组织再生。需要可靠的烧伤模型来阐明局部事件的确切顺序，以便能够更好地预测局部炎症何时触发全身炎症过程。与其他体外皮肤培养方法不同，我们使用新鲜的腹部皮肤外植体来引入接触性烧伤。组织学和超微结构分析证实为部分厚度烧伤病理。分析烧伤后24小时局部炎症、热休克反应和组织再生的关键介质的基因表达谱和细胞因子产生谱。我们发现，烧伤后不久，参与组织再生和炎症的因子的表达显著增加。为了研究纯粹的炎症反应，我们将脂多糖注射到真皮中。与烧伤相比，内毒素注射启动了炎症反应，而热休克和组织再生基因的表达模式在实验期间没有受到影响。这种新颖的体外人体皮肤模型适合于研究烧伤等皮肤损伤的局部早期反应，同时保持完整的整体组织结构，并为烧伤后伤口愈合过程一开始的局部机制提供有价值的见解。
Burn injuries initiate numerous processes such as heat shock response, inflammation and tissue regeneration. Reliable burn models are needed to elucidate the exact sequence of local events to be able to better predict when local inflammation triggers systemic inflammatory processes. In contrast to other ex vivo skin culture approaches, we used fresh abdominal skin explants to introduce contact burn injuries. Histological and ultrastructural analyses confirmed a partial-thickness burn pathology. Gene expression patterns and cytokine production profiles of key mediators of the local inflammation, heat shock response, and tissue regeneration were analyzed for 24 h after burn injury. We found significantly increased expression of factors involved in tissue regeneration and inflammation soon after burn injury. To investigate purely inflammation-mediated reactions we injected lipopolysaccharide into the dermis. In comparison to burn injury, lipopolysaccharide injection initiated an inflammatory response while expression patterns of heat shock and tissue regeneration genes were unaffected for the duration of the experiment. This novel ex vivo human skin model is suitable to study the local, early responses to skin injuries such as burns while maintaining an intact overall tissue structure and it gives valuable insights into local mechanisms at the very beginning of the wound healing process after burn injuries.
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影响因子：
3.8
作者：
Finnerty, Celeste C.;Przkora, Rene;Herndon, David N.;Jeschke, Marc G.
通讯作者：
Jeschke, Marc G.
影响因子：
2.7
作者：
Ederer, Ines A.;Hacker, Stefan;Pauzenberger, Reinhard
通讯作者：
Pauzenberger, Reinhard
影响因子：
2.7
作者：
Bergquist, Maria;Hastbacka, Johanna;Lipcsey, Miklos
通讯作者：
Lipcsey, Miklos
影响因子：
3
作者：
Companjen, AR;van der Wel, LI;Prens, EP
通讯作者：
Prens, EP
DOI：
10.1016/s0140-6736(16)31406-4
发表时间：
2016-10-01
期刊：
Lancet (London, England)
影响因子：
--
作者：
Finnerty CC;Jeschke MG;Branski LK;Barret JP;Dziewulski P;Herndon DN
通讯作者：
Herndon DN