Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains: most of the genetic differences are localized in six regions.
Genome analysis of Treponema pallidum subsp. pallidum and subsp. pertenue strains: most of the genetic differences are localized in six regions.
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DOI：
10.1371/journal.pone.0015713
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发表时间：
2010-12-29
期刊：
影响因子：
3.7
通讯作者：
Šmajs D
中科院分区：
文献类型：
--
作者：
Mikalová L;Strouhal M;Čejková D;Zobaníková M;Pospíšilová P;Norris SJ;Sodergren E;Weinstock GM;Šmajs D
包括 T. p. 在内的八种密螺旋体的基因组。梅毒株（Nichols、SS14、DAL-1 和 Mexico A），T. p.在 133 个重叠扩增子中扩增了 Pertenue 菌株（Samoa D、CDC-2 和 Gauthier）和 Fribourg-Blanc 分离株，并对这些片段的限制性模式进行了比较。基于全基因组指纹（WGF）分析的基因组的大致大小范围为 1139.3–1140.4 kb，同源基因组区域中的估计基因组序列一致性为 99.57–99.98%。限制性目标位点分析检测了参考 Nichols 基因组中是否存在 1773 个单独的限制性位点，揭示了所有菌株的基因组结构高度相似性。未分类的猿猴 Fribourg-Blanc 分离株与 T. p. 关系更密切。比 T. p. 更持久。梅毒株。 T. p. 之间的大部分遗传差异。苍白球和 T. p.永久菌株在六个基因组区域中积累。这些基因组差异可能导致观察到的 T. p. 之间的致病性差异。苍白球和 T. p.永久菌株。这些序列差异区域可用于梅毒和雅司病菌株的分子检测和区分。
The genomes of eight treponemes including T. p. pallidum strains (Nichols, SS14, DAL-1 and Mexico A), T. p. pertenue strains (Samoa D, CDC-2 and Gauthier), and the Fribourg-Blanc isolate, were amplified in 133 overlapping amplicons, and the restriction patterns of these fragments were compared. The approximate sizes of the genomes investigated based on this whole genome fingerprinting (WGF) analysis ranged from 1139.3–1140.4 kb, with the estimated genome sequence identity of 99.57–99.98% in the homologous genome regions. Restriction target site analysis, detecting the presence of 1773 individual restriction sites found in the reference Nichols genome, revealed a high genome structure similarity of all strains. The unclassified simian Fribourg-Blanc isolate was more closely related to T. p. pertenue than to T. p. pallidum strains. Most of the genetic differences between T. p. pallidum and T. p. pertenue strains were accumulated in six genomic regions. These genome differences likely contribute to the observed differences in pathogenicity between T. p. pallidum and T. p. pertenue strains. These regions of sequence divergence could be used for the molecular detection and discrimination of syphilis and yaws strains.
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影响因子：
4.2
作者：
Coil DA;Vandersmissen L;Ginevra C;Jarraud S;Lammertyn E;Anné J
通讯作者：
Anné J
影响因子：
3.8
作者：
Harper, Kristin N.;Ocampo, Paolo S.;Steiner, Bret M.;George, Robert W.;Silverman, Michael S.;Bolotin, Shelly;Pillay, Allan;Saunders, Nigel J.;Armelagos, George J.
通讯作者：
Armelagos, George J.
影响因子：
3.1
作者：
Cox, David L.;Luthra, Amit;Radolf, Justin D.
通讯作者：
Radolf, Justin D.
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
6.4
作者：
Centurion-Lara, A;Castro, C;Lukehart, SA
通讯作者：
Lukehart, SA