Optimizing optical flow cytometry for cell volume-based sorting and analysis.
Optimizing optical flow cytometry for cell volume-based sorting and analysis.
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DOI：
10.1371/journal.pone.0016053
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发表时间：
2011-01-20
期刊：
影响因子：
3.7
通讯作者：
Kirschner MW
中科院分区：
文献类型：
--
作者：
Tzur A;Moore JK;Jorgensen P;Shapiro HM;Kirschner MW
细胞大小是细胞功能和最终组织结构的核心定义特征。对不同大小的细胞亚群进行分类的能力将有助于在基因组和蛋白质组水平上研究细胞获得和保持其大小的机制。然而，目前可用的细胞分选器不能直接以电子方式测量细胞体积，因此，希望知道在这种仪器中可以进行的光学测量中的哪一种能够提供对体积的最佳估计。我们研究了几种不同细胞系中的几种不同的光散射和荧光测量，从分布的高端和低端对细胞部分进行分类，并通过电子测量体积来确定哪种分选策略产生了最好的分离体积分布。因为我们发现不同的光学测量对不同的细胞系是最佳的，我们建议遵循这一程序将使其他研究人员能够优化他们自己的细胞分选器，以基于体积来分离他们所处理的细胞类型。
Cell size is a defining characteristic central to cell function and ultimately to tissue architecture. The ability to sort cell subpopulations of different sizes would facilitate investigation at genomic and proteomic levels of mechanisms by which cells attain and maintain their size. Currently available cell sorters, however, cannot directly measure cell volume electronically, and it would therefore be desirable to know which of the optical measurements that can be made in such instruments provide the best estimate of volume. We investigated several different light scattering and fluorescence measurements in several different cell lines, sorting cell fractions from the high and low end of distributions, and measuring volume electronically to determine which sorting strategy yielded the best separated volume distributions. Since we found that different optical measurements were optimal for different cell lines, we suggest that following this procedure will enable other investigators to optimize their own cell sorters for volume-based separation of the cell types with which they work.
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影响因子：
48
作者：
Godin, Michel;Delgado, Francisco Feijo;Son, Sungmin;Grover, William H.;Bryan, Andrea K.;Tzur, Amit;Jorgensen, Paul;Payer, Kris;Grossman, Alan D.;Kirschner, Marc W.;Manalis, Scott R.
通讯作者：
Manalis, Scott R.
影响因子：
64.8
作者：
Martin, Sophie G.;Berthelot-Grosjean, Martine
通讯作者：
Berthelot-Grosjean, Martine
影响因子：
64.8
作者：
Moseley, James B.;Mayeux, Adeline;Nurse, Paul
通讯作者：
Nurse, Paul
影响因子：
56.9
作者：
FULWYLER, MJ
通讯作者：
FULWYLER, MJ
DOI：
10.1039/c39830000787
发表时间：
1983-01-01
影响因子：
--
作者：
NAKAZAKI, M;YAMAMOTO, K;OKAMOTO, Y
通讯作者：
OKAMOTO, Y