Proanthocyanidins' efficacy in stabilizing dentin collagen against enzymatic degradation: MALDI-TOF and FTIR analyses.
Proanthocyanidins' efficacy in stabilizing dentin collagen against enzymatic degradation: MALDI-TOF and FTIR analyses.
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DOI：
10.1016/j.jdent.2013.03.007
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发表时间：
2013-06
影响因子：
4.4
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Liu Y;Wang Y
鉴于胶原蛋白样品的大小和形状以及应用 PA 的方式均与临床相关，研究葡萄籽提取物原花青素 (PA) 在酶促环境中改善牙本质胶原蛋白可持续性的能力。人牙本质被切成 6 μm 厚的薄膜。在 35 wt% 磷酸中脱盐 15 s 后，薄膜分别在 0%（对照）、0.5%、1%、2%、3.75%、7.5% 和 15%（w/w）的 PA 浓度下进行 30 s 的处理。然后将膜在 0.1 wt% 胶原酶中消化 1 小时和 24 小时。通过 MALDI-TOF 质谱法测定液体消化物中降解的胶原蛋白的量。通过ATR-FTIR分析PA掺入牙本质胶原蛋白的趋势。对照在 1 小时内表现出完全消化。相比之下，用 0.5% 和 1% PA 处理的胶原蛋白在消化的前 1 小时内分别产生了 13.84 ± 4.69% 和不可检测的降解水平，在接下来的 23 小时内分别产生了额外的 17.48 ± 4.38% 和 4.50 ± 1.68%。无论消化时间如何，用 ≥2 wt% PA 处理的胶原蛋白都不会被显着消化。 FTIR 光谱显示 PA 掺入量在 ≥2 wt% PA 时达到饱和。 2 wt% 及以上的 PA 处理 30 秒可以为牙本质胶原蛋白提供最佳保护，防止胶原酶消化。这项研究证明了当以临床相关方式应用时，PA 在稳定脱矿牙本质胶原蛋白方面具有非凡的效率，并确定了其使用的最佳条件。
To investigate grape seed extract proanthocyanidins’ (PA) capability in improving dentin collagen’s sustainability in an enzymatic environment, given that the size and shape of the collagen samples, and the manner to apply PA are both clinically relevant. Human dentin was sectioned into 6-μm-thick films. After demineralization in 35 wt% phosphoric acid for 15 s, the films were subject to 30 s of treatment at PA concentrations of 0% (control), 0.5%, 1%, 2%, 3.75%, 7.5% and 15% (w/w), respectively. The films were then digested in 0.1 wt% collagenase for 1 h and 24 h. The amount of degraded collagen in the liquid digests was determined by MALDI-TOF mass spectroscopy. The trend of PA’s incorporation into dentin collagen was analyzed by ATR-FTIR. The control exhibited complete digestion in 1 h. In contrast, collagen treated with 0.5% and 1% PA afforded 13.84 ± 4.69% and an undetectable level of degradation, respectively in the first 1 h of digestion, and additional 17.48 ± 4.38% and 4.50 ± 1.68%, respectively in the following 23 h. Collagen treated with ≥2 wt% PA was not significantly digested regardless of digestion time. FTIR spectroscopy revealed that PA incorporation was saturated at ≥2 wt% PA. Thirty seconds of PA treatment at 2 wt% and above could provide optimal protection for dentin collagen against collagenase digestion. This study demonstrated PA’s extraordinary efficiency in stabilizing demineralized dentin collagen when it is applied in a clinical relevant manner, and identified the optimal conditions for its utilization.
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影响因子：
2.9
作者：
JAMALL, IS;FINELLI, VN;HEE, SSQ
通讯作者：
HEE, SSQ
影响因子：
14
作者：
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通讯作者：
Leventouri, Theodora
影响因子：
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作者：
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通讯作者：
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影响因子：
4.4
作者：
Green B;Yao X;Ganguly A;Xu C;Dusevich V;Walker MP;Wang Y
通讯作者：
Wang Y
DOI：
10.1002/jbm.b.31759
发表时间：
2011-02
影响因子：
3.4
作者：
Xu, Changqi;Wang, Yong
通讯作者：
Wang, Yong