Grape seed proanthocyanidins increase collagen biodegradation resistance in the dentin/adhesive interface when included in an adhesive.
Grape seed proanthocyanidins increase collagen biodegradation resistance in the dentin/adhesive interface when included in an adhesive.
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DOI：
10.1016/j.jdent.2010.08.004
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发表时间：
2010-11
影响因子：
4.4
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Green B;Yao X;Ganguly A;Xu C;Dusevich V;Walker MP;Wang Y
牙本质粘结的当代方法可以产生含有空隙和暴露的脱矿胶原纤维的混合层（HL）。原花青素（PA）已被证明可以交联和强化脱矿牙本质胶原，但其对HL内胶原降解的影响尚未得到广泛研究。本研究的目的是比较在酶促胶原降解条件下，添加和不添加葡萄籽提取物PA的BisGMA/HEMA模型粘合剂所产生的HL的形态差异。模型粘合剂配制有和没有5%PA粘结到酸蚀牙本质。用Goldner三色染色法对从粘合样本上切下的5 μ m厚切片进行染色。然后将标本暴露于0.1%胶原酶溶液0、1或6天。胶原酶处理后，用SEM/TEM分析标本。染色未显示用两种粘合剂产生的HL的差异。SEM显示，在所有胶原酶处理条件下，用含PA的粘合剂粘合的样本均存在完整的胶原原纤维。在相同的胶原酶处理后，在用粘合剂粘合而没有PA的标本中没有观察到这些完整的胶原纤维。透射电镜证实，含有PA的标本在用胶原酶溶液处理后仍显示正常的胶原纤维组织和尺寸。相比之下，无PA的标本经胶原酶处理后，界面区中的无序胶原原纤维缺乏正常胶原的典型交叉带。牙科粘合剂中葡萄籽提取物PA的存在可能会抑制HL内未受保护的胶原纤维的生物降解。
Contemporary methods of dentin bonding could create hybrid layers (HLs) containing voids and exposed, demineralized collagen fibers. Proanthocyanidins (PA) have been shown to crosslink and strengthen demineralized dentin collagen, but their effects on collagen degradation within the HL have not been widely studied. The purpose of this study was to compare the morphological differences of HLs created by BisGMA/HEMA model adhesives with and without the addition of grape seed extract PA under conditions of enzymatic collagen degradation. Model adhesives formulated with and without 5% PA were bonded to the acid etched dentin. Five-μm-thick sections cut from the bonded specimens were stained with Goldner’s trichrome. The specimens were then exposed to 0.1% collagenase solution for zero, one, or six days. Following collagenase treatment, the specimens were analyzed with SEM/TEM. Staining did not reveal a difference in the HLs created with the two adhesives. SEM showed the presence of intact collagen fibrils in all collagenase treatment conditions for specimens bonded with adhesive containing PA. These integral collagen fibrils were not observed in the specimens bonded with adhesive without PA after the same collagenase treatment. TEM confirmed that the specimens containing PA still showed normal collagen fibril organization and dimensions after treatment with collagenase solution. In contrast, disorganized collagen fibrils in the interfacial zone lacked the typical cross-banding of normal collagen after collagenase treatment for specimens without PA. The presence of grape seed extract PA in dental adhesives may inhibit the biodegradation of unprotected collagen fibrils within the HL.
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DOI：
10.1002/jbm.b.31095
发表时间：
2009-02
影响因子：
3.4
作者：
Kostoryz, Elisabet L.;Dharmala, Kiran;Ye, Qiang;Wang, Yong;Huber, Jesse;Park, Jong-Gu;Snider, Grant;Katz, J. Lawrence;Spencer, Paulette
通讯作者：
Spencer, Paulette
DOI：
10.1002/jmb.b.31022
发表时间：
2008-08-01
影响因子：
3.4
作者：
Bedran-Russo, Ana Karina B.;Pashley, David H.;Miescke, Klaus J.
通讯作者：
Miescke, Klaus J.
影响因子：
1.9
作者：
Ganss, Carolina;Schlueter, Nadine;Klimek, Joachim
通讯作者：
Klimek, Joachim
DOI：
10.1002/jbm.b.31417
发表时间：
2009-10
影响因子：
3.4
作者：
Al-Ammar, Aiman;Drummond, James L.;Bedran-Russo, Ana Karina
通讯作者：
Bedran-Russo, Ana Karina
影响因子：
3.5
作者：
Song, SE;Choi, BK;Kim, CK
通讯作者：
Kim, CK