Enhancement in dentin collagen's biological stability after proanthocyanidins treatment in clinically relevant time periods.
Enhancement in dentin collagen's biological stability after proanthocyanidins treatment in clinically relevant time periods.
复制标题
DOI：
10.1016/j.dental.2013.01.013
复制
发表时间：
2013-04
期刊：
影响因子：
5
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Liu, Yi;Chen, Mingsheng;Yao, Xiaomei;Xu, Changqi;Zhang, Ying;Wang, Yong
目的：研究原花青素(PA)是否能够在临床相关的时间段内通过交联提高牙本质胶原蛋白的生物稳定性。用3.75wt%PA溶液处理脱矿牙本质胶原块10 S、1min、30min、60min、120min、360min和720min。用0.1%的胶原酶在37℃下消化2 h、6 h、12 h、24 h、36 h和48 h，计算消化后的失重率，以评价PA处理的胶原的抗酶降解能力。用傅里叶变换红外光谱(FTIR)研究了PA处理不同时间后，PA与胶原的相互作用。胶原酶消化试验表明，PA处理10 S可增强胶原蛋白对酶刺激的抵抗力。傅立叶变换红外光谱进一步证实，无论处理时间如何，PA确实被结合到胶原中，可能是通过PA和胶原之间的化学作用的机制。本研究证实，PA能有效地交联胶原，并在短至10 S的时间内提高其生物稳定性。因此，使用PA作为起始剂在临床上是可行的，是提高现有牙本质粘结系统耐久性的一种有前途的方法。
To investigate whether proanthocyanidins (PA) is capable of improving dentin collagen’s biological stability through cross-linking within time periods that are clinically relevant. Demineralized dentin collagen slabs were treated with 3.75 wt% PA solution for 10 s, 1 min, 30 min, 60 min, 120 min, 360 min, and 720 min, respectively. The resultant cross-linked collagen samples were subject to digestion with 0.1% collagenase at 37 °C for 2 h, 6 h, 12 h, 24 h, 36 h, and 48 h. The percentage of weight loss after digestion was calculated to evaluate PA-treated collagen’s resistance toward enzymatic degradation. Fourier-Transformed Infrared (FTIR) spectroscopy was used to probe evidences of PA-collagen interactions after various periods of PA treatment. The collagenase digestion assay suggests that PA treatment as short as 10 s can enhance collagen’s resistance toward enzymatic challenge. The FTIR spectroscopy further verifies that PA is indeed incorporated into collagen regardless of treatment time, possibly via a mechanism involving the chemical interactions between PA and collagen. This study confirmed that PA can effectively cross-link collagen and improve its biological stability in time periods as short as 10 s. The use of PA as a priming agent is therefore clinically feasible and is a promising approach to improving the durability of current dentin bonding systems.
登录
查看更多内容
DOI：
10.1016/j.jmbbm.2011.05.003
发表时间：
2011-10
影响因子：
3.9
作者：
Castellan, Carina Strano;Bedran-Russo, Ana Karina;Karol, Sachin;Rodrigues Pereira, Patricia Nobrega
通讯作者：
Rodrigues Pereira, Patricia Nobrega
影响因子：
7.6
作者：
Macedo, G. V.;Yamauchi, M.;Bedran-Russo, A. K.
通讯作者：
Bedran-Russo, A. K.
影响因子：
2.9
作者：
Baxter, NJ;Lilley, TH;Williamson, MP
通讯作者：
Williamson, MP
影响因子：
4.4
作者：
Green B;Yao X;Ganguly A;Xu C;Dusevich V;Walker MP;Wang Y
通讯作者：
Wang Y
DOI：
10.1002/jmb.b.31022
发表时间：
2008-08-01
影响因子：
3.4
作者：
Bedran-Russo, Ana Karina B.;Pashley, David H.;Miescke, Klaus J.
通讯作者：
Miescke, Klaus J.