Citrullination in the periodontium--a possible link between periodontitis and rheumatoid arthritis.
Citrullination in the periodontium--a possible link between periodontitis and rheumatoid arthritis.
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DOI：
10.1007/s00784-015-1556-7
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发表时间：
2016-05
影响因子：
3.4
通讯作者：
Eick, Sigrun
中科院分区：
文献类型：
--
作者：
Laugisch, Oliver;Wong, Alicia;Sroka, Aneta;Kantyka, Tomasz;Koziel, Joanna;Neuhaus, Klaus;Sculean, Anton;Venables, Patrick J.;Potempa, Jan;Moeller, Burkhard;Eick, Sigrun
关键词：
本研究的目的是评估人类和细菌的肽基精氨酸脱亚胺酶（PAD）的活性，在龈沟液（GCF）的背景下，抗瓜氨酸抗原决定簇的抗体在类风湿性关节炎和牙周炎的血清水平。在52例类风湿关节炎（RA）患者（48例牙周炎和4例无牙周炎）和44例非RA对照（28例牙周炎和16例无牙周炎）的GCF中测定人PAD和牙龈卟啉单胞菌衍生酶（PPAD）活性。通过ELISA测定抗瓜氨酸化表位的血清抗体。牙周炎相关细菌用核酸法检测。瓜氨酸存在于26例（50%）RA患者和23例（48%）对照中。PAD和PPAD活性分别在36例（69%）和30例（58%）RA患者中检测到，分别在30例（68%）和21例（50%）对照中检测到。PPAD活性在患有牙周炎的RA和非RA患者中高于那些没有牙周炎的患者（p = 0.038; p = 0.004），并且在59个牙龈卟啉单胞菌阳性样本中的35个中检测到，并且在37个牙龈卟啉单胞菌阴性样本中的16个中检测到，这与针对该物种的高抗体水平有关。牙周组织中的PAD和PPAD活性在RA和非RA牙周炎患者中升高。由驻留在上皮细胞中的牙龈卟啉单胞菌分泌的PPAD可在牙周组织的远距离区域或甚至远距离组织中发挥其瓜氨酸化活性。在牙周炎中，人和细菌肽基精氨酸脱亚胺酶对蛋白质/肽的瓜氨酸酶可能在破坏易感个体的免疫耐受性后产生抗体。
The aim of the present study was to assess human and bacterial peptidylarginine deiminase (PAD) activity in the gingival crevicular fluid (GCF) in the context of serum levels of antibodies against citrullinated epitopes in rheumatoid arthritis and periodontitis. Human PAD and Porphyromonas gingivalis-derived enzyme (PPAD) activities were measured in the GCF of 52 rheumatoid arthritis (RA) patients (48 with periodontitis and four without) and 44 non-RA controls (28 with periodontitis and 16 without). Serum antibodies against citrullinated epitopes were measured by ELISA. Bacteria being associated with periodontitis were determined by nucleic-acid based methods. Citrullination was present in 26 (50%) RA patients and 23 (48%) controls. PAD and PPAD activities were detected in 36 (69%) and 30 (58%) RA patients, respectively, and in 30 (68%) and 21 (50%) controls, respectively. PPAD activity was higher in RA and non-RA patients with periodontitis than in those without (p = 0.038; p = 0.004), and was detected in 35 of 59 P. gingivalis positive samples, and in in 16 of 37 P. gingivalis-negative samples in association with high antibody levels against that species. PAD and PPAD activities within the periodontium are elevated in RA and non-RA patients with periodontitis. PPAD secreted by P. gingivalis residing in epithelial cells may exert its citrullinating activity in distant regions of the periodontium or even distant tissues. In periodontitis, the citrullination of proteins/peptides by human and bacterial peptidylarginine deiminases may generate antibodies after breaching immunotolerance in susceptible individuals.
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DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
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DOI：
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