Infection of Nigrospora nonsegmented RNA Virus 1 Has Important Biological Impacts on a Fungal Host.
Infection of Nigrospora nonsegmented RNA Virus 1 Has Important Biological Impacts on a Fungal Host.
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黑孢子菌不分段 RNA 病毒 1 的感染对真菌宿主具有重要的生物学影响
DOI：
10.3390/v14040795
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发表时间：
2022-04-12
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
Nigrospora nonsegmented RNA virus 1（NoNRV 1）是一种存在于黑孢霉（Nigrospora）中的病毒，但其对宿主的生物学效应尚不清楚。本研究从我国北方地区分离到一株N.从菊花叶中分离到的病毒，并确定了NoNRV 1感染的分离株。本研究所鉴定的NoNRV 1基因组序列与已报道的NoNRV 1分离株HN-21的基因组序列高度同源，因此我们将新分离的NoNRV 1暂命名为NoNRV 1-ZJ。病毒唑的药物处理成功地从菌株9-1中去除了NoNRV 1-ZJ，这为我们提供了一个理想的对照，以确定NoNRV 1感染对宿主真菌的生物学影响。通过比较病毒携带（9-1）和病毒治愈（9-1C）的菌株，我们的结果表明，感染NoNRV 1促进宿主细胞的色素沉着，而它没有明显的影响，在马铃薯葡萄糖琼脂平板上的主机生长时，受到渗透或氧化胁迫。有趣的是，我们观察到病毒感染对N.寄主真菌对棉花叶片的致病性。总的来说，我们的工作为N.包括色素沉着、毒力低下和耐热性。
Nigrospora nonsegmented RNA virus 1 (NoNRV1) has been reported previously in the fungus Nigrospora oryzae, but its biological effects on its host are unknown. In this work, we isolated a strain 9-1 of N. oryzae from a chrysanthemum leaf and identified NoNRV1 infection in the isolated strain. The genome sequence of NoNRV1 identified here is highly homologous to that of the isolate HN-21 of NoNRV1 previously reported; thus, we tentatively designated the newly identified NoNRV1 as NoNRV1-ZJ. Drug treatment with Ribavirin successfully removed NoNRV1-ZJ from the strain 9-1, which provided us with an ideal control to determine the biological impacts of NoNRV1 infection on host fungi. By comparing the virus-carrying (9-1) and virus-cured (9-1C) strains, our results indicated that infection with NoNRV1 promoted the pigmentation of the host cells, while it had no discernable effects on host growth on potato dextrose agar plates when subjected to osmotic or oxidative stress. Interestingly, we observed inhibitory impacts of virus infection on the thermotolerance of N. oryzae and the pathogenicity of the host fungus in cotton leaves. Collectively, our work provides clear evidence of the biological relevance of NoNRV1 infection in N. oryzae, including pigmentation, hypovirulence, and thermotolerance.
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影响因子：
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DOI：
10.3390/jof4020054
发表时间：
2018-05-05
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Liu C;Cleckler B;Morsy M
通讯作者：
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