TOM1 family conservation within the plant kingdom for tobacco mosaic virus accumulation.
TOM1 family conservation within the plant kingdom for tobacco mosaic virus accumulation.
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DOI：
10.1111/mpp.13375
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发表时间：
2023-11
影响因子：
4.9
通讯作者：
Hu, Qun
中科院分区：
文献类型：
--
作者：
Zhang, Hui;Hu, Qun
烟草花叶病毒（tobacco mosaic virus，TMV）的易感因子TOM 1（Tobamovirus MULTIPLICATION 1）是烟草花叶病毒（tobacco mosaic virus，TMV）有效增殖所必需的。虽然已经进行了一些系统发育和功能分析的TOM 1家族成员，全面分析的TOM 1同源物的基础上，从最古老的植物界内最年轻的代表，分析的支持病毒的积累和相互作用与其他主机和病毒蛋白还没有报道。本研究以本氏烟草（Nicotianabenthamiana）和烟草花叶病毒（TMV）为模型系统，对烟草烟草中的TOM 1同源基因进行了功能分析。benthamiana和来自不同植物谱系的其它植物物种。我们修改了一个多重基因组编辑工具，并产生了一个六重突变体，其中TMV增殖显着抑制。我们发现，来自N. benthamiana表现出可变的能力，以支持TMV增殖。进化分析表明，TOM 1家族仅限于植物界，可能起源于绿藻门，表明TOM 1家族的起源古老。我们发现，TOM 1家族获得的能力，促进TMV增殖后，分歧的苔藓和穗苔。此外，来自不同植物物种的TOM 1直系同源物促进TMV增殖的能力以及TOM 1和TOM 2A之间以及TOM 1和TMV编码的复制蛋白之间的相互作用是高度保守的，表明TOM 2A-TOM 1-TMV Hel模块在促进TMV增殖中的保守性质。我们的研究不仅揭示了促进烟草花叶病毒增殖的基因模块的保守性，而且为抗TMV作物的开发提供了有价值的策略。TOM 1家族具有古老的起源，其促进烟草花叶病毒增殖和与病毒复制复合物的其他组分相互作用的能力在植物界中高度保守。
The susceptibility factor TOBAMOVIRUS MULTIPLICATION 1 (TOM1) is required for efficient multiplication of tobacco mosaic virus (TMV). Although some phylogenetic and functional analyses of the TOM1 family members have been conducted, a comprehensive analysis of the TOM1 homologues based on phylogeny from the most ancient to the youngest representatives within the plant kingdom, analysis of support for tobamovirus accumulation and interaction with other host and viral proteins has not been reported. In this study, using Nicotiana benthamiana and TMV as a model system, we functionally characterized the TOM1 homologues from N. benthamiana and other plant species from different plant lineages. We modified a multiplex genome editing tool and generated a sextuple mutant in which TMV multiplication was dramatically inhibited. We showed that TOM1 homologues from N. benthamiana exhibited variable capacities to support TMV multiplication. Evolutionary analysis revealed that the TOM1 family is restricted to the plant kingdom and probably originated in the Chlorophyta division, suggesting an ancient origin of the TOM1 family. We found that the TOM1 family acquired the ability to promote TMV multiplication after the divergence of moss and spikemoss. Moreover, the capacity of TOM1 orthologues from different plant species to promote TMV multiplication and the interactions between TOM1 and TOM2A and between TOM1 and TMV‐encoded replication proteins are highly conserved, suggesting a conserved nature of the TOM2A–TOM1–TMV Hel module in promoting TMV multiplication. Our study not only revealed a conserved nature of a gene module to promote tobamovirus multiplication, but also provides a valuable strategy for TMV‐resistant crop development. The TOM1 family has an ancient origin, and its ability to promote tobacco mosaic virus multiplication and interact with other components of the virus replication complex is highly conserved in the plant kingdom.
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影响因子：
6.7
作者：
Ishibashi K;Mawatari N;Miyashita S;Kishino H;Meshi T;Ishikawa M
通讯作者：
Ishikawa M
DOI：
10.1073/pnas.79.19.5818
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GOELET, P;LOMONOSSOFF, GP;KARN, J
通讯作者：
KARN, J
影响因子：
3.5
作者：
Asano, M;Satoh, R;Ishikawa, M
通讯作者：
Ishikawa, M
影响因子：
7.4
作者：
Ishikawa, Masayuki;Yoshida, Tetsuya;Matsuyama, Momoko;Kouzai, Yusuke;Kano, Akihito;Ishibashi, Kazuhiro
通讯作者：
Ishibashi, Kazuhiro
影响因子：
3.5
作者：
Ding, XS;Liu, JZ;Nelson, RS
通讯作者：
Nelson, RS