Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology.
Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology.
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DOI：
10.1002/cncr.26349
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发表时间：
2012-02-15
期刊：
影响因子：
6.2
通讯作者：
Iqbal SM
中科院分区：
文献类型：
--
作者：
Wan Y;Mahmood MA;Li N;Allen PB;Kim YT;Bachoo R;Ellington AD;Iqbal SM
检测少量循环肿瘤细胞是重要的，特别是在癌症的早期阶段。CTC的数量很少，很难检测，因为很少有方法足够敏感，可以将这些细胞与其他细胞区分开来。鉴于CTC在体内的稀疏性，改善选择性表面官能化分子的亲和力是重要的。有许多蛋白质和适体提供了如此高的亲和力，但使用表面纳米纹理甚至进一步增加了这种亲和力。这项工作报告了一种方法，以提高亲和力的肿瘤细胞捕获，通过使用新的适体对细胞膜过表达的表皮生长因子受体（EGFR）的纳米纹理的聚二甲基硅氧烷（PDMS）基板。表面固定的适体用于从生理样品中特异性捕获肿瘤细胞。PDMS的纳米织构化增加了纳米级的表面粗糙度。这增加了有效表面积并导致显著更高程度的表面官能化。该现象导致固定的EGFR特异性RNA适体分子的密度增加，并提供了从混合物中捕获癌细胞的显著更高的效率。数据显示CTC可以被捕获和富集，导致更高的产率，但背景更高。载玻片、普通PDMS和用适体官能化的纳米纹理PDMS的比较表明，在纳米纹理PDMS上使用适体的双重方法对于癌症细胞学装置可以是重要因素，特别是对于芯片上实验室朝向捕获效率的更高产率的想法。
Detection of a small number of circulating tumor cells is important, especially at the early stages of cancer. The small number of CTCs is hard to detect as very few approaches are sensitive enough to differentiate these from the pool of other cells. Improving the affinity of a selective surface-functionalized molecule is important given the sparsity of CTCs in vivo. There are a number of proteins and aptamers that provide such a high affinity but using a surface nano-texturing increases this affinity even further. This work reports an approach to improve affinity of tumor cell capture by using novel aptamers against cell-membrane over-expressed Epidermal Growth Factor Receptors (EGFR) on a nano-textured polydimethylsiloxane (PDMS) substrate. Surface immobilized aptamers are used to specifically capture tumor cells from physiological samples. The nano-texturing of PDMS increased surface roughness at the nanoscale. This increased the effective surface area and resulted in a significantly higher degree of surface functionalization. The phenomenon resulted in increased density of immobilized EGFR specific RNA aptamer molecules and provided significantly higher efficiency to capture cancer cells from a mixture. The data showed that CTCs could be captured and enriched leading to higher yield, yet higher background. The comparison of glass slides, plain PDMS and nano-textured PDMS functionalized with aptamers show that a two-fold approach of using aptamers on nano-textured PDMS can be an important factor for cancer cytology devices especially for the idea of lab-on-chip towards higher yield in capture efficiency.
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影响因子：
14
作者：
Miller, DC;Thapa, A;Webster, TJ
通讯作者：
Webster, TJ
影响因子：
6.2
作者：
Fuentes, M;Mateo, C;Fern치ndez-Lafuente, R
通讯作者：
Fern치ndez-Lafuente, R
影响因子：
7.8
作者：
Osborne, SE;Matsumura, I;Ellington, AD
通讯作者：
Ellington, AD
影响因子：
15
作者：
Adams, Andre A.;Okagbare, Paul I.;Soper, Steven A.
通讯作者：
Soper, Steven A.
影响因子：
2.3
作者：
Bhattacharya, Shantanu;Singh, Rajeev Kr.;Gangopadhyay, Shubhra
通讯作者：
Gangopadhyay, Shubhra