Natural DNA variation at candidate loci is associated with potato chip color, tuber starch content, yield and starch yield.
Natural DNA variation at candidate loci is associated with potato chip color, tuber starch content, yield and starch yield.
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DOI：
10.1007/s00122-008-0746-y
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发表时间：
2008-05
影响因子：
5.4
通讯作者：
Gebhardt, Christiane
中科院分区：
文献类型：
--
作者：
Li, Li;Paulo, Maria-Joao;Strahwald, Josef;Luebeck, Jens;Hofferbert, Hans-Reinhard;Tacke, Eckhart;Junghans, Holger;Wunder, Joerg;Draffehn, Astrid;van Eeuwijk, Fred;Gebhardt, Christiane
植物的种子、果实、块茎和根的淀粉和糖含量等复杂性状受多种遗传和环境因素控制。了解它们的分子基础将有助于在作物改良计划（“精确育种”）中诊断和组合上级等位基因。基于候选基因的关联遗传学是实现这一目标的一种方法。对四倍体马铃薯品种和家系选育无性系进行了2 a的冷藏前后片品质、块茎淀粉含量、产量和淀粉产量的测定。薯片品质与块茎含糖量呈负相关。对243个个体的11条马铃薯染色体上的总共36个位点进行了自然DNA变异评估。这些基因座包括微卫星和编码在碳水化合物代谢或运输中起作用的酶的基因（候选基因座）。这些标记被用来分析群体结构，并与块茎品质性状进行关联性检验。标记与1-4个性状的高度显著和稳健的关联被鉴定。最常见的是与芯片质量和块茎淀粉含量。增加块茎淀粉含量的等位基因提高了薯片品质，反之亦然。除了两个例外，观察到最显著和最强的关联（q < 0.01）与编码淀粉和糖代谢或运输酶的基因中的DNA变异有关。比较基因座间的连锁和连锁不平衡，为所分析的育种材料中存在大的单倍型块提供了证据。本文的在线版本（doi：10.1007/s 00122 -008-0746-y）包含补充材料，可供授权用户使用。
Complex characters of plants such as starch and sugar content of seeds, fruits, tubers and roots are controlled by multiple genetic and environmental factors. Understanding their molecular basis will facilitate diagnosis and combination of superior alleles in crop improvement programs (“precision breeding”). Association genetics based on candidate genes is one approach toward this goal. Tetraploid potato varieties and breeding clones related by descent were evaluated for 2 years for chip quality before and after cold storage, tuber starch content, yield and starch yield. Chip quality is inversely correlated with tuber sugar content. A total of 36 loci on 11 potato chromosomes were evaluated for natural DNA variation in 243 individuals. These loci included microsatellites and genes coding for enzymes that function in carbohydrate metabolism or transport (candidate loci). The markers were used to analyze population structure and were tested for association with the tuber quality traits. Highly significant and robust associations of markers with 1–4 traits were identified. Most frequent were associations with chip quality and tuber starch content. Alleles increasing tuber starch content improved chip quality and vice versa. With two exceptions, the most significant and robust associations (q < 0.01) were observed with DNA variants in genes encoding enzymes that function in starch and sugar metabolism or transport. Comparing linkage and linkage disequilibrium between loci provided evidence for the existence of large haplotype blocks in the breeding materials analyzed. The online version of this article (doi:10.1007/s00122-008-0746-y) contains supplementary material, which is available to authorized users.
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影响因子：
3.3
作者：
Breseghello, F;Sorrells, ME
通讯作者：
Sorrells, ME
影响因子：
2.9
作者：
Berloo, R. van;Hutten, R. C. B.;van Eck, H. J.
通讯作者：
van Eck, H. J.
DOI：
10.1007/bf02364726
发表时间：
1969-01-01
期刊：
European Potato Journal
影响因子：
--
作者：
BURTON W G
通讯作者：
BURTON W G
影响因子：
3.5
作者：
Huang, SW;Vleeshouwers, VGAA;Jacobsen, E
通讯作者：
Jacobsen, E
影响因子：
7.4
作者：
PRESSEY, R;SHAW, R
通讯作者：
SHAW, R