Nucleotide sequence of v-fps in the PRCII strain of avian sarcoma virus-猫眼课题宝

禽肉瘤病毒PRCII株v-fps的核苷酸序列

DOI：

10.1128/jvi.50.1.125-131.1984

发表时间：

1984

期刊：

Journal of Virology

影响因子：

5.4

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

C. C. Huang;C. Hammond;J. Bishop

PRCII是一种鸟类逆转录病毒，其癌基因（v-fps）可诱导鸟类纤维肉瘤。病毒基因v-fps是通过转导细胞基因c-fps的一个未知部分而产生的。PRCII是弱致癌性相比，藤波肉瘤病毒，另一种转化病毒含有v-fps。作为阐明PRCII毒力降低的分子基础的第一步，我们已经确定了PRCII基因组中v-fps的整个核苷酸序列。PRCII中的v-fps结构域编码分子量约为1000的多肽。60，500融合到由病毒结构基因gag编码的多蛋白的一部分。PRCII的杂合gag-fps多聚蛋白将具有约1000的分子量。98，100，与先前对PRCII基因组编码的蛋白质的研究结果雅阁。Fujinami肉瘤病毒和PRCII中fps和gag之间的连接点位于fps中的相同位置，但位于gag中的不同位置。一个1,020个核苷酸的序列，以6个核苷酸的直接重复为界，存在于Fujinami肉瘤病毒的v-fps中，但不存在于PRCII中。我们的数据应允许进一步探索的结构和功能之间的关系，在转化蛋白编码的v-fps。

PRCII is an avian retrovirus whose oncogene (v-fps) induces fibrosarcomas in birds. The viral gene v-fps arose by transduction of an undetermined portion of a cellular gene known as c-fps. PRCII is weakly oncogenic when compared with Fujinami sarcoma virus, another transforming virus containing v-fps. As a first step in the elucidation of the molecular basis for the decreased virulence of PRCII, we have determined the entire nucleotide sequence of v-fps in the PRCII genome. The v-fps domain in PRCII encodes a polypeptide with a molecular weight of ca. 60,500 fused to a portion of the polyprotein encoded by the viral structural gene gag. The hybrid gag-fps polyprotein of PRCII would have a molecular weight of ca. 98,100, in accord with results of previous studies of the protein encoded by the PRCII genome. The leftward junctions between fps and gag in Fujinami sarcoma virus and PRCII are located at the same position in fps, but at different positions in gag. A sequence of 1,020 nucleotides, bounded by direct repeats of 6 nucleotides, is present in v-fps of Fujinami sarcoma virus but absent from PRCII. Our data should permit further explorations of the relationship between structure and function in the transforming protein encoded by v-fps.