Population structure and transmission dynamics of Plasmodium vivax in the Republic of Korea based on microsatellite DNA analysis.
Population structure and transmission dynamics of Plasmodium vivax in the Republic of Korea based on microsatellite DNA analysis.
复制标题
DOI：
10.1371/journal.pntd.0001592
复制
发表时间：
2012
影响因子：
3.8
通讯作者：
Kano S
中科院分区：
文献类型：
--
作者：
Iwagami M;Fukumoto M;Hwang SY;Kim SH;Kho WG;Kano S
为了控制疟疾，了解每个流行地区寄生虫的遗传结构是重要的。间日疟原虫广泛分布于亚洲和南美洲的热带至温带地区，但尚未制定有效的消除策略。例如，在韩国，当地的间日疟疾在20世纪70年代末被消灭，但从1993年开始重新出现。我们利用微卫星DNA标记估计了韩国间日疟原虫的种群结构和传播的时间动态。基于10个高度多态性的间日疟原虫基因组微卫星DNA位点，我们分析了1994年至2008年收集的255个韩国间日疟原虫分离株。获得了87株分离株的等位基因数据，并结合等位基因数据确定了其微卫星单倍型。共观察到40个单倍型。主要有两种单倍型：H16和H25。1996 - 2005年检出H16菌株9株（10%），1995 - 2003年检出H25菌株27株（31%）。这些结果表明，间日疟原虫在温带国家韩国的重组率低于在热带地区的重组率，在热带地区很少见到相同的单倍型。接下来，我们通过eBURST分析估计了40个单倍型之间的关系。发现了两个主要群体：一个群体由36株（41%）组成，包括H25；其余20株（23%），包括H16。尽管重组率很低，但自1997年以来，也观察到与这两组不同的其他新的单倍型（H27）。这些结果表明间日疟原虫持续从其他人群来源（可能是朝鲜）传入。利用间日疟种群微卫星DNA的分子流行病学对评估疟原虫的种群结构和传播动态是有效的，这些信息有助于在流行地区消除间日疟。间日疟疾广泛流行，主要在亚洲和南美洲，2009年报告了3.9亿例病例。同年，全世界有28.5亿人处于危险之中。间日疟原虫不仅在热带和亚热带地区流行，而且在寒冷季节没有蚊子的温带地区也流行。当大多数疟疾研究人员将研究重点放在热带地区的间日疟原虫上时，我们利用疟原虫基因组中的10个高度多态性微卫星DNA（短串联重复DNA序列）对韩国（温带地区）间日疟原虫的特征进行了暂时检测，并强调了热带和温带种群之间的差异。我们发现，与已报道的热带间日疟种群相比，韩国间日疟种群的遗传多样性和重组率较低。我们还发现，从1994年到2008年，种群中的一些寄生虫克隆发生了变化，这表明寄生虫不断从其他种群引入，可能来自朝鲜。间日疟原虫的多态DNA标记是估计其在流行地区传播情况的有用工具。
In order to control malaria, it is important to understand the genetic structure of the parasites in each endemic area. Plasmodium vivax is widely distributed in the tropical to temperate regions of Asia and South America, but effective strategies for its elimination have yet to be designed. In South Korea, for example, indigenous vivax malaria was eliminated by the late 1970s, but re-emerged from 1993. We estimated the population structure and temporal dynamics of transmission of P. vivax in South Korea using microsatellite DNA markers. We analyzed 255 South Korean P. vivax isolates collected from 1994 to 2008, based on 10 highly polymorphic microsatellite DNA loci of the P. vivax genome. Allelic data were obtained for the 87 isolates and their microsatellite haplotypes were determined based on a combination of allelic data of the loci. In total, 40 haplotypes were observed. There were two predominant haplotypes: H16 and H25. H16 was observed in 9 isolates (10%) from 1996 to 2005, and H25 in 27 (31%) from 1995 to 2003. These results suggested that the recombination rate of P. vivax in South Korea, a temperate country, was lower than in tropical areas where identical haplotypes were rarely seen in the following year. Next, we estimated the relationships among the 40 haplotypes by eBURST analysis. Two major groups were found: one composed of 36 isolates (41%) including H25; the other of 20 isolates (23%) including H16. Despite the low recombination rate, other new haplotypes that are genetically distinct from the 2 groups have also been observed since 1997 (H27). These results suggested a continual introduction of P. vivax from other population sources, probably North Korea. Molecular epidemiology using microsatellite DNA of the P. vivax population is effective for assessing the population structure and transmission dynamics of the parasites - information that can assist in the elimination of vivax malaria in endemic areas. Vivax malaria is widely prevalent, mainly in Asia and South America with 390 million reported cases in 2009. Worldwide, in the same year, 2.85 billion people were at risk. Plasmodium vivax is prevalent not only in tropical and subtropical areas but also in temperate areas where there are no mosquitoes in cold seasons. While most malaria researchers are focusing their studies on the parasite in tropical areas, we examined the characteristics of P. vivax in South Korea (temperate area) temporally, using 10 highly polymorphic microsatellite DNA (a short tandem repeat DNA sequence) in the parasite genome, and highlighted the differences between the tropical and temperate populations. We found that the South Korean P. vivax population had low genetic diversity and low recombination rates in comparison to tropical P. vivax populations that had been reported. We also found that some of the parasite clones in the population were changing from 1994 to 2008, evidence suggesting the continual introduction of the parasite from other populations, probably from North Korea. Polymorphic DNA markers of the P. vivax parasite are useful tools for estimating the situation of its transmission in endemic areas.
登录
查看更多内容
影响因子：
10.7
作者：
Imwong, M;Sudimack, D;Anderson, TJC
通讯作者：
Anderson, TJC
影响因子：
10.7
作者：
Jongwutiwes, S;Putaporntip, C;Hughes, AL
通讯作者：
Hughes, AL
影响因子：
3.2
作者：
Feil, EJ;Li, BC;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
10.7
作者：
Joy, Deirdre A.;Gonzalez-Ceron, Lilia;Su, Xin-zhuan
通讯作者：
Su, Xin-zhuan
影响因子：
3.8
作者：
Guerra CA;Howes RE;Patil AP;Gething PW;Van Boeckel TP;Temperley WH;Kabaria CW;Tatem AJ;Manh BH;Elyazar IR;Baird JK;Snow RW;Hay SI
通讯作者：
Hay SI