Altered Amniotic Fluid Levels of Hyaluronic Acid in Fetal Rats with Myelomeningocele: Understanding Spinal Cord Injury.
Altered Amniotic Fluid Levels of Hyaluronic Acid in Fetal Rats with Myelomeningocele: Understanding Spinal Cord Injury.
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脊髓脊膜膨出胎鼠羊水透明质酸水平的改变：了解脊髓损伤。
DOI：
10.1089/neu.2018.5894
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发表时间：
2019
影响因子：
4.2
通讯作者：
Krynska,Barbara
中科院分区：
文献类型：
--
作者：
Zieba,Jolanta;Walczak,Maciej;Gordiienko,Oleg;Gerstenhaber,JonathanA;Smith,GeorgeM;Krynska,Barbara
脊髓膜脑膨出(MMC)是一种破坏性的先天性神经管缺陷，导致脊髓暴露于宫内环境，导致继发性脊髓损伤和严重损伤。虽然继发性发病机制在临床上是相关的，但宫内获得性脊髓损伤的确切原因仍不清楚。本研究的目的是确定维甲酸诱导的丝裂霉素C模型羊水中透明质酸(HA)浓度是否与正常对照组不同，以及这些差异是否会对羊水粘度产生影响。我们的数据显示，在胚胎第18天(E18)和E20天，患有MMC的胎儿房颤(MMC-AF)和正常对照组(Norm-AF)的HA浓度没有显著差异。此后，Norm-AF组HA浓度显著升高，而MMC-AF组则无明显变化。与Norm-AF相比，在E21时，MMC-AF中HA的浓度和MMC-AF的粘度显著降低。琼脂糖凝胶电泳证实，在E21时，MMC-AF的HA水平显著低于Norm-AF。在MMC-AF中未检测到HA降解活性。综上所述，我们发现MMC胎鼠的房颤HA水平和房颤粘度存在缺陷。这些数据与低水平HA导致的房颤粘度降低在机械创伤加重MMC损伤部位脊髓损伤中可能起到的作用有关。
Myelomeningocele (MMC) is a devastating congenital neural tube defect that results in the exposure of spinal cord to the intrauterine environment, leading to secondary spinal cord injury and severe impairment. Although the mechanisms underlying the secondary pathogenesis are clinically relevant, the exact cause ofin utero–acquired spinal cord damage remains unclear. The objective of this study was to determine whether the hyaluronic acid (HA) concentration in amniotic fluid (AF) in the retinoic acid-induced model of MMC is different from that in normal controls and whether these differences could have an impact on the viscosity of AF. Our data shows that the concentration of HA in AF samples from fetuses with MMC (MMC-AF) and normal control samples (Norm-AF) were not significantly different at embryonic day 18 (E18) and E20. Thereafter, the HA concentration significantly increased in Norm-AF but not in MMC-AF. Compared with Norm-AF, the concentration of HA in MMC-AF and the viscosity of MMC-AF were significantly lower at E21. Agarose gel electrophoresis confirmed a significant reduction in the HA level of MMC-AF compared with Norm-AF at E21. No HA-degrading activity was detected in MMC-AF. In summary, we identified a deficiency in the AF level of HA and the viscosity of AF in fetal rats with MMC. These data are discussed in relation to a potential role the reduction in the AF viscosity due to the low level of HA may play in the exacerbating effects of mechanical trauma on spinal cord damage at the MMC lesion site.
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影响因子：
2.9
作者：
LEE, HG;COWMAN, MK
通讯作者：
COWMAN, MK
影响因子：
27.4
作者：
D. Swann;E. Radin;M. Nazimiec;P. A. Weisser;Nancy Curran;George LEWINNEKt
通讯作者：
D. Swann;E. Radin;M. Nazimiec;P. A. Weisser;Nancy Curran;George LEWINNEKt
动物模型中胎儿脊髓脊膜膨出修复的组织工程策略
影响因子：
2.2
作者：
Miho Watanabe;Aimee G. Kim;A. Flake
通讯作者：
A. Flake
DOI：
10.1016/0885-4505(86)90077-0
发表时间：
1986-04-01
期刊：
BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY
影响因子：
--
作者：
DAHL, LB;DAHL, IMS;BORRESEN, AL
通讯作者：
BORRESEN, AL
DOI：
10.1016/0006-2944(83)90018-2
发表时间：
1983
期刊：
Biochemical medicine
影响因子：
--
作者：
L. Dahl;J. Hopwood;U. Laurent;K. Lilja;A. Tengblad
通讯作者：
A. Tengblad