Serum antibodies to Porphyromonas gingivalis chaperone HtpG predict health in periodontitis susceptible patients.
Serum antibodies to Porphyromonas gingivalis chaperone HtpG predict health in periodontitis susceptible patients.
复制标题
DOI：
10.1371/journal.pone.0001984
复制
发表时间：
2008-04-23
期刊：
影响因子：
3.7
通讯作者：
Lopatin DE
中科院分区：
文献类型：
--
作者：
Shelburne CE;Shelburne PS;Dhople VM;Sweier DG;Giannobile WV;Kinney JS;Coulter WA;Mullally BH;Lopatin DE
伴侣蛋白是一种普遍存在的保守蛋白，在先天性和适应性免疫中对新蛋白的稳定、缺陷蛋白和免疫显性抗原的修复/去除至关重要。牙周病是一种慢性炎症性感染，与牙龈卟啉单胞菌感染有关，最终导致牙齿支撑结构的破坏。我们以前报道的血清抗体与人类伴侣Hsp 90在牙龈炎，一种可逆的形式的牙龈疾病局限于口腔软组织反应的研究。在这些研究中，口腔健康状况最好的受试者的抗体水平最高。我们假设牙龈卟啉单胞菌HSP 90同源物（HtpG）的抗体可能与破坏性牙周炎的保护/抵抗有关。使用克隆的HtpG和肽抗原的ELISA测定证实了被牙龈卟啉单胞菌定殖的牙龈炎受试者具有较高的血清抗HtpG水平，并且伴随着较低的附着丧失水平。此外，健康受试者的牙龈卟啉单胞菌HtpG蛋白血清抗体水平高于慢性或侵袭性牙周炎患者。我们发现牙齿附着丧失与抗牙龈卟啉单胞菌HtpG水平呈负相关（p = 0.043），但与抗具核梭杆菌（一种口腔机会致病菌）HtpG水平无关。 此外，在治疗前抗牙龈卟啉单胞菌HtpG水平较高的受试者中，对牙周治疗的反应更成功（p = 0.018）。 与抗F无类似关系。核内HtpG水平。当用牙龈卟啉单胞菌HtpG区域的合成肽重复这些实验时，获得了类似的结果。我们的研究结果表明：1）抗牙龈卟啉单胞菌HtpG抗体是保护性的，因此预测健康的牙周炎易感患者; 2）可以增强宿主对牙周炎的防御; 3）牙龈卟啉单胞菌HtpG的独特肽提供了作为有效诊断靶标和疫苗候选物的显著潜力。这些结果与一种与细菌直接结合无关的新型免疫控制机制相一致。
Chaperones are ubiquitous conserved proteins critical in stabilization of new proteins, repair/removal of defective proteins and immunodominant antigens in innate and adaptive immunity. Periodontal disease is a chronic inflammatory infection associated with infection by Porphyromonas gingivalis that culminates in the destruction of the supporting structures of the teeth. We previously reported studies of serum antibodies reactive with the human chaperone Hsp90 in gingivitis, a reversible form of gingival disease confined to the oral soft tissues. In those studies, antibodies were at their highest levels in subjects with the best oral health. We hypothesized that antibodies to the HSP90 homologue of P. gingivalis (HtpG) might be associated with protection/resistance against destructive periodontitis. ELISA assays using cloned HtpG and peptide antigens confirmed gingivitis subjects colonized with P. gingivalis had higher serum levels of anti-HtpG and, concomitantly, lower levels of attachment loss. Additionally, serum antibody levels to P. gingivalis HtpG protein were higher in healthy subjects compared to patients with either chronic or aggressive periodontitis. We found a negative association between tooth attachment loss and anti-P. gingivalis HtpG (p = 0.043) but not anti-Fusobacterium nucleatum (an oral opportunistic commensal) HtpG levels. Furthermore, response to periodontal therapy was more successful in subjects having higher levels of anti-P. gingivalis HtpG before treatment (p = 0.018). There was no similar relationship to anti-F. nucleatum HtpG levels. Similar results were obtained when these experiments were repeated with a synthetic peptide of a region of P. gingivalis HtpG. Our results suggest: 1) anti-P. gingivalis HtpG antibodies are protective and therefore predict health periodontitis-susceptable patients; 2) may augment the host defence to periodontitis and 3) a unique peptide of P. gingivalis HtpG offers significant potential as an effective diagnostic target and vaccine candidate. These results are compatible with a novel immune control mechanism unrelated to direct binding of bacteria.
登录
查看更多内容
DOI：
10.1164/rccm.200702-291oc
发表时间：
2007-10-01
影响因子：
24.7
作者：
Chatterjee, Anuran;Dimitropoulou, Christiana;Catravas, John D.
通讯作者：
Catravas, John D.
影响因子：
3.5
作者：
Mullally, BH;Dace, B;Coulter, WA
通讯作者：
Coulter, WA
影响因子：
6.7
作者：
BAGRAMIAN, RA;FARGHALY, MM;POMERVILLE, JL
通讯作者：
POMERVILLE, JL
影响因子：
5.8
作者：
Kozarov, Emil;Sweier, Domenica;Lopatin, Dennis
通讯作者：
Lopatin, Dennis
影响因子：
6.7
作者：
Huszti, Z;Bene, L;Prohászka, Z
通讯作者：
Prohászka, Z