Bioprinting of a Blue Light-Cross-Linked Biodegradable Hydrogel Encapsulating Amniotic Mesenchymal Stem Cells for Intrauterine Adhesion Prevention.
Bioprinting of a Blue Light-Cross-Linked Biodegradable Hydrogel Encapsulating Amniotic Mesenchymal Stem Cells for Intrauterine Adhesion Prevention.
复制标题
DOI：
10.1021/acsomega.1c02117
复制
发表时间：
2021-09-14
期刊：
影响因子：
4.1
通讯作者：
Han L
中科院分区：
文献类型：
--
作者：
Feng M;Hu S;Qin W;Tang Y;Guo R;Han L
宫腔粘连（IUA）是子宫手术后常见的并发症，可导致月经量减少、闭经、周期性下腹痛、不孕等临床症状，将三维打印的水凝胶放置于损伤部位与邻近组织之间，可作为物理屏障防止粘连，在愈合过程中隔离损伤区域。本研究采用三维生物打印技术构建了不同比例的甲基丙烯酸化明胶（GelMA）和甲基丙烯酸化胶原（ColMA）复合水凝胶负载羊膜间充质干细胞（AMSCs）的组织水凝胶。与单一的GelMA水凝胶相比，复合防粘连水凝胶（GelMA/ColMA）表现出适当的溶胀比，增强的机械性能，和令人印象深刻的稳定性。同时，GelMA/ColMA复合水凝胶的微观结构显示出更加致密、相互连通的微孔结构。此外，细胞毒性研究表明，GelMA/ColMA水凝胶对AMSC具有细胞相容性。最后，研究了干细胞包封水凝胶的制备，并成功制备了连续释放7天以上的干细胞，且细胞功能正常。体内实验结果表明，GelMA/ColMA/hAMSC（人羊膜间充质干细胞）水凝胶可以防止大鼠IUA模型中的腔粘连。因此，生物打印蓝光交联的生物可降解水凝胶具有令人满意的抗宫腔粘连效果，具有优异的物理性能和生物相容性，可用作宫腔粘连的预防屏障。
Intrauterine adhesion (IUA) is a common and prevailing complication after uterine surgery, which can lead to clinical symptoms such as a low menstrual volume, amenorrhea, periodic lower abdominal pain, infertility, and so on. Placing a three-dimensional printing hydrogel between the injured site and the adjacent tissue is considered to be a physical barrier to prevent adhesion, which can isolate the damaged area during the healing process. In this work, a tissue hydrogel with various proportions of a methacrylated gelatin (GelMA) and methacrylated collagen (ColMA) composite hydrogel loaded with amniotic mesenchymal stem cells (AMSCs) was constructed by using three-dimensional biological printing technology. Compared with the single GelMA hydrogel, the composite antiadhesion hydrogel (GelMA/ColMA) showed an appropriate swelling ratio, enhanced mechanical properties, and impressive stability. Meanwhile, the microstructure of the GelMA/ColMA composite hydrogel showed a denser and interconnected microporous structure. In addition, the cytotoxicity study indicated that the GelMA/ColMA hydrogel has a cytocompatibility nature toward AMSCs. Finally, the fabrication of stem cell encapsulation hydrogels was studied, and the cells could be released continuously for more than 7 days with the normal cell function. The results of in vivo experiments indicated that the GelMA/ColMA/hAMSC (human amnion mesenchymal stem cell) hydrogel can prevent cavity adhesion in a rat IUA model. Therefore, bioprinting a biodegradable hydrogel cross-linked by blue light has satisfactory anticavity adhesion effects with excellent physical properties and biocompatibility, which could be used as a preventive barrier for intrauterine adhesion.
登录
查看更多内容
影响因子：
6.7
作者：
Gil-Sanchis, Claudia;Cervello, Irene;Simon, Carlos
通讯作者：
Simon, Carlos
影响因子：
11.2
作者：
Cai, Xianqun;Hu, Shengxue;Shi, Xianai
通讯作者：
Shi, Xianai
DOI：
10.1016/j.ijbiomac.2020.12.011
发表时间：
2021-01-15
影响因子：
8.2
作者：
Dutta, Sayan Deb;Hexiu, Jin;Lim, Ki-Taek
通讯作者：
Lim, Ki-Taek
DOI：
10.1016/j.ijbiomac.2018.09.122
发表时间：
2019-02-15
影响因子：
8.2
作者：
Hu, Shengxue;Cai, Xianqun;Shi, Xianai
通讯作者：
Shi, Xianai
Circ-100290 通过 miR-449a/eNOS 和 miR-449a/VEGFA 轴正向调节人羊膜来源间充质干细胞条件培养基诱导的血管生成
影响因子：
9.2
作者：
Tang, Zichun;Wu, Xiaoyue;Yuan, Xiaoqin
通讯作者：
Yuan, Xiaoqin