Extracts of Tectona grandis and Vernonia amygdalina have anti-Toxoplasma and pro-inflammatory properties in vitro.
Extracts of Tectona grandis and Vernonia amygdalina have anti-Toxoplasma and pro-inflammatory properties in vitro.
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DOI：
10.1051/parasite/2018014
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Gbeassor M
中科院分区：
文献类型：
--
作者：
Dégbé M;Debierre-Grockiego F;Tété-Bénissan A;Débare H;Aklikokou K;Dimier-Poisson I;Gbeassor M
柚木（柚木）和苦叶斑鸠菊（Vernonia amygdalina）是西非传统医药中使用的植物。在这项研究中，我们测试了乙醇和水乙醇提取物的树皮和叶的T。grandis和V. amygdalina叶的乙醇提取物对弓形虫（一种引起弓形虫病的原生动物寄生虫）的抑制作用。苦杏仁叶乙醇提取物中酚类、鞣质、黄酮类和多糖的含量均与苦杏仁叶乙醇提取物中酚类、鞣质、黄酮类和多糖的含量相当。该提取物对T.弓形虫，对哺乳动物细胞的细胞毒性最低，但与其他植物提取物相比具有中等的抗氧化活性。T. grandis树皮也具有升高的抗T。弓形虫活性，对哺乳动物细胞的低细胞毒性，以及最高的抗氧化活性之一。然而，这种提取物的植物化学成分与水乙醇提取物没有很大的不同，水乙醇提取物没有抗T。弓形虫活动此外，苦杏仁叶的乙醇提取物具有较强的抗氧化活性，而苦杏仁叶的乙醇提取物不具有抗氧化活性。结果表明，巨树皮能显著增加抗原提呈细胞产生TNF-α和NO的能力。这两种提取物有降低抗原呈递细胞表面主要组织相容性复合物分子表达的趋势，而它们不调节凋亡细胞的百分比。信号通路的研究将有助于确定这些植物提取物的作用机制。
Tectona grandis (teak) and Vernonia amygdalina (bitter leaf) are plants used in traditional medicine in West Africa. In this study, we tested ethanolic and hydro-ethanolic extracts of bark and leaves of T. grandis and ethanolic extract of leaves of V. amygdalina for their inhibitory effect on Toxoplasma gondii, a protozoan parasite responsible for toxoplasmosis. Ethanolic extract of V. amygdalina leaves had proportional contents of phenols, tannins, flavonoids, and polysaccharides. This extract presented the highest efficacy against T. gondii, the lowest cytotoxicity to mammalian cells, but moderate anti-oxidant activity compared to other plant extracts. Ethanolic extract of T. grandis bark also had elevated anti-T. gondii activity, low cytotoxicity on mammalian cells, and one of the highest anti-oxidant activities. However, the phytochemical content of this extract was not very different from the hydro-ethanolic extract, which had no anti-T. gondii activity. In addition, ethanolic extract of V. amygdalina leaves, but not of T. grandis bark, significantly increased the production of TNF-α and NO by antigen-presenting cells. Both extracts had the tendency to decrease expression of major histocompatibility complex molecules at the surface of antigen-presenting cells, while they did not modulate the percentage of apoptotic cells. A study of signalling pathways would help to determine the mechanisms of action of these plant extracts.
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影响因子：
3.6
作者：
Adedapo, Adeolu Alex;Aremu, Olujoke Janet;Oyagbemi, Ademola Adetokunbo
通讯作者：
Oyagbemi, Ademola Adetokunbo
DOI：
10.3390/molecules19055898
发表时间：
2014-05-07
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
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影响因子：
4.3
作者：
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通讯作者：
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影响因子：
11.2
作者：
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通讯作者：
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影响因子：
3.2
作者：
Debierre-Grockiego, Francoise;Molitor, Nicole;Luder, Carsten G K
通讯作者：
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