Co-circulation of alpha- and beta-coronaviruses in Pteropus vampyrus flying foxes from Indonesia.
Co-circulation of alpha- and beta-coronaviruses in Pteropus vampyrus flying foxes from Indonesia.
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来自印度尼西亚的翼展Vampyrus飞狐中α-和β-核纳病毒的共同循环。
DOI：
10.1111/tbed.14762
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发表时间：
2022-11
影响因子：
4.3
通讯作者：
Mendenhall, Ian H. H.
中科院分区：
文献类型：
--
作者：
Ch'ng, Lena;Tsang, Susan M. M.;Ong, Zoe A. A.;Low, Dolyce H. W.;Wiantoro, Sigit;Smith, Ina L. L.;Simmons, Nancy B. B.;Su, Yvonne C. F.;Lohman, David J. J.;Smith, Gavin J. D.;Mendenhall, Ian H. H.
蝙蝠是α和β冠状病毒的重要宿主。冠状病毒（CoV）已在几个东南亚国家的翼足类蝙蝠中检测到，但对印度尼西亚群岛的冠状病毒与其哺乳动物生物多样性的比例知之甚少。在这项研究中，我们筛选混合粪便样本从印度尼西亚殖民地的狐蝠属。无偏的下一代测序检测到与Rousetus leschenaultii CoV HKU 9和Eidolon helvum CoV相关的蝙蝠CoV。使用常规半巢式泛冠状病毒PCR检测进一步筛查121份粪便样本。3份阳性样本成功测序，系统发育重建显示存在α和β冠状病毒。在一个单一的P. vampyus栖息地中检测到属于Nobecovirus、Decacovirus和Pedacovirus亚属的CoV。这项研究扩展了印度尼西亚狐蝠冠状病毒多样性的现有知识，强调了对殖民地进行纵向监测的必要性，因为持续的城市化和森林砍伐增加了溢出事件的风险。
Bats are important reservoirs for alpha- and beta-coronaviruses. Coronaviruses (CoV) have been detected in pteropodid bats from several Southeast Asian countries, but little is known about coronaviruses in the Indonesian archipelago in proportion to its mammalian biodiversity. In this study, we screened pooled fecal samples from Indonesian colonies of Pteropus spp. with unbiased next generation sequencing. Bat CoVs related to Rousettus leschenaultii CoV HKU9 and Eidolon helvum CoV were detected. The 121 fecal samples were further screened using a conventional hemi-nested pan-coronavirus PCR assay. Three positive samples were successfully sequenced, and phylogenetic reconstruction revealed the presence of alpha- and beta-coronaviruses. CoVs belonging to the subgenera Nobecovirus, Decacovirus and Pedacovirus were detected in a single P. vampyrus roost. This study expands current knowledge of coronavirus diversity in Indonesian flying foxes, highlighting the need for longitudinal surveillance of colonies as continuing urbanisation and deforestation heighten the risk of spillover events.
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影响因子：
4.3
作者：
Kudagammana, H. D. W. S.;Thevanesam, V.;Noordeen, F.
通讯作者：
Noordeen, F.
影响因子：
--
作者：
Corman VM;Muth D;Niemeyer D;Drosten C
通讯作者：
Drosten C
中国多种蝙蝠α冠状病毒和β冠状病毒的鉴定为冠状病毒相关疾病的进化和起源提供了新的见解
影响因子：
5.2
作者：
Han, Yelin;Du, Jiang;Jin, Qi
通讯作者：
Jin, Qi
影响因子：
2.6
作者：
Homwong N;Jarvis MC;Lam HC;Diaz A;Rovira A;Nelson M;Marthaler D
通讯作者：
Marthaler D
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM