Comparative transcriptome analysis of silkworm, Bombyx mori colleterial gland suggests their functional role in mucous secretion.
Comparative transcriptome analysis of silkworm, Bombyx mori colleterial gland suggests their functional role in mucous secretion.
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家蚕、家蚕结肠腺的比较转录组分析表明它们在粘液分泌中的功能作用
DOI：
10.1371/journal.pone.0198077
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Wei G
中科院分区：
文献类型：
--
作者：
Yang L;Gao Q;Dai J;Yuan G;Wang L;Qian C;Zhu B;Liu C;Wei G
家蚕成年雌性个体体内存在粘液腺（CG），它有助于将卵粘附在寄主植物表面。尽管这个器官已被认知了几个世纪，但只有其形态和分泌物得到了研究。它们的基因表达谱和生理作用在很大程度上仍然未知。借助高通量下一代测序（NGS）技术，我们报道了对从家蚕H9和P50品系中分离出的粘液腺进行的比较转录组分析。分别从H9和P50品系的粘液腺中获得了总共19,896,957和20,446,366条干净读数；然后进行了差异表达分析，鉴定出1509个差异表达基因（DEGs）。其中，与H9个体相比，P50个体中有1001个基因上调，508个基因下调。差异表达基因的基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析证实，许多差异表达基因与“氨基酸转运和代谢”、“核苷酸转运和代谢”以及“无机离子转运和代谢”相关，25个与“细胞外基质 - 受体相互作用通路”、“鞘脂代谢通路”和“氨基糖和核苷糖代谢通路”相关的差异表达基因可能参与粘液腺发育和粘液分泌过程。根据这些数据，我们假设粘液腺在为胶质分泌物形成提供有利的生理环境方面起着重要作用。此外，对粘液腺中差异表达基因的基因本体论富集分析和差异表达分析表明，该腺体可能参与将糖类、离子、氨基酸和核苷糖等小溶质运输到粘液腺。我们的研究结果为进一步研究粘液腺功能奠定了基础。
Colleterial glands (CG) present in the body of adult female of Bombyx mori, which can help adhere eggs on the surface of the host plants. Although this organ has been known for centuries, only morphology and its secretions have been studied. Their gene expression profiles and physiological roles remain largely unknown. Aided by high-throughput next generation sequencing (NGS), we reported the comparative transcriptome analysis of CG isolated from the H9 and the P50 strains of Bombyx mori. A total of 19,896,957 and 20,446,366 clean reads were obtained from CG of H9 and the P50 strains, respectively; then differential expression analysis was performed, and 1,509 differentially expressed genes (DEGs) were identified. Among them, 1,001 genes are up-regulated and 508 genes are down-regulated in P50 individuals compared with H9 individuals. The enrichment of GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) of DEGs confirmed that many DEGs were associated with “Amino acid transport and metabolism”, “Nucleotide transport and metabolism”, and “Inorganic ion transport and metabolism”, 25 of the DEGs related to the “ECM-receptor interaction passway”, “sphingolipid metabolism passway”, and “amino sugar and nucleotide sugar metabolism passway” were potentially involved in the process of CG development and mucus secretion. According to these data, we hypothesized that CG play an important role in providing favorable physiological environment for the glue secretion formation. In addition, GO enrichment and differential expression analysis of the DEGs in the CG indicate that this gland may be involved in the transporting of small solutes such as sugars, ions, amino acids and nucleotide sugar to the CG. Our findings lay the foundation for further research on CG function.
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影响因子：
4.4
作者：
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DOI：
10.1099/00221287-137-2-323
发表时间：
1991-02-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
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作者：
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通讯作者：
VUORIO, O
影响因子：
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作者：
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通讯作者：
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