Mechanistic strategies of microbial communities regulating lignocellulose deconstruction in a UK salt marsh.
Mechanistic strategies of microbial communities regulating lignocellulose deconstruction in a UK salt marsh.
复制标题
英国盐沼中微生物群落调节木质纤维素分解的机制策略。
DOI：
10.1186/s40168-020-00964-0
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发表时间：
2021-02-17
期刊：
影响因子：
15.5
通讯作者：
Bruce NC
中科院分区：
文献类型：
--
作者：
Leadbeater DR;Oates NC;Bennett JP;Li Y;Dowle AA;Taylor JD;Alponti JS;Setchfield AT;Alessi AM;Helgason T;McQueen-Mason SJ;Bruce NC
Salt marshes are major natural repositories of sequestered organic carbon with high burial rates of organic matter, produced by highly productive native flora. Accumulated carbon predominantly exists as lignocellulose which is metabolised by communities of functionally diverse microbes. However, the organisms that orchestrate this process and the enzymatic mechanisms employed that regulate the accumulation, composition and permanence of this carbon stock are not yet known. We applied meta-exo-proteome proteomics and 16S rRNA gene profiling to study lignocellulose decomposition in situ within the surface level sediments of a natural established UK salt marsh. Our studies revealed a community dominated by Gammaproteobacteria, Bacteroidetes and Deltaproteobacteria that drive lignocellulose degradation in the salt marsh. We identify 42 families of lignocellulolytic bacteria of which the most active secretors of carbohydrate-active enzymes were observed to be Prolixibacteracea, Flavobacteriaceae, Cellvibrionaceae, Saccharospirillaceae, Alteromonadaceae, Vibrionaceae and Cytophagaceae. These families secreted lignocellulose-active glycoside hydrolase (GH) family enzymes GH3, GH5, GH6, GH9, GH10, GH11, GH13 and GH43 that were associated with degrading Spartina biomass. While fungi were present, we did not detect a lignocellulolytic contribution from fungi which are major contributors to terrestrial lignocellulose deconstruction. Oxidative enzymes such as laccases, peroxidases and lytic polysaccharide monooxygenases that are important for lignocellulose degradation in the terrestrial environment were present but not abundant, while a notable abundance of putative esterases (such as carbohydrate esterase family 1) associated with decoupling lignin from polysaccharides in lignocellulose was observed. Here, we identify a diverse cohort of previously undefined bacteria that drive lignocellulose degradation in the surface sediments of the salt marsh environment and describe the enzymatic mechanisms they employ to facilitate this process. Our results increase the understanding of the microbial and molecular mechanisms that underpin carbon sequestration from lignocellulose within salt marsh surface sediments in situ and provide insights into the potential enzymatic mechanisms regulating the enrichment of polyphenolics in salt marsh sediments. Video Abstract The online version contains supplementary material available at 10.1186/s40168-020-00964-0.
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影响因子：
5
作者：
Cortes-Tolalpa L;Norder J;van Elsas JD;Falcao Salles J
通讯作者：
Falcao Salles J
影响因子：
4.5
作者：
BENNER, R;FOGEL, ML;SPRAGUE, EK
通讯作者：
SPRAGUE, EK
影响因子：
2.6
作者：
Bouchard, V.;Creach, V.;Mariotti, A.
通讯作者：
Mariotti, A.
影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
影响因子：
7.8
作者：
Cragg SM;Beckham GT;Bruce NC;Bugg TD;Distel DL;Dupree P;Etxabe AG;Goodell BS;Jellison J;McGeehan JE;McQueen-Mason SJ;Schnorr K;Walton PH;Watts JE;Zimmer M
通讯作者：
Zimmer M