Bacterial killing by light-triggered release of silver from biomimetic metal nanorods.
Bacterial killing by light-triggered release of silver from biomimetic metal nanorods.
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DOI：
10.1002/smll.201301283
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发表时间：
2014-01-15
期刊：
影响因子：
13.3
通讯作者：
Messersmith, Phillip B.
中科院分区：
文献类型：
--
作者：
Black, Kvar C. L.;Sileika, Tadas S.;Yi, Ji;Zhang, Ran;Rivera, Jose G.;Messersmith, Phillip B.
关键词：
在等离子体共振下照射贵金属纳米颗粒导致大量的热产生，并且由这种能量转换产生的瞬时和高度局部化的温度升高可以通过等离子体加热结合到细胞表面的金纳米棒（NR）而用于光热治疗。在这里，我们报告了第一次使用等离子体激元加热从靶向细菌细胞壁的金核/银壳（Au@Ag）NR局部释放银。采用一种新的仿生方法制备Au@Ag核-壳纳米核，包括在Au NR表面沉积一层薄的有机聚多巴胺（PD）底漆，然后进行自发的化学镀银金属化，并结合抗菌抗体和钝化聚合物以靶向革兰氏阴性和革兰氏阳性细菌。S. epidermidis和E.由于等离子体激元加热和银释放的组合抗菌作用，在暴露于光时观察到用Au@Ag NR靶向的大肠杆菌细胞。抗菌效果远大于单独的等离子体加热或银，这意味着细胞靶向的等离子体加热和照射后相关的银释放之间的强治疗协同作用。我们的研究结果表明，当与光照射结合时，Au@Ag NR具有潜在的抗菌用途，这在以前没有描述过。
Illumination of noble metal nanoparticles at the plasmon resonance causes substantial heat generation, and the transient and highly localized temperature increases that result from this energy conversion can be exploited for photothermal therapy by plasmonically heating gold nanorods (NRs) bound to cell surfaces. Here, we report the first use of plasmonic heating to locally release silver from gold core/silver shell (Au@Ag) NRs targeted to bacterial cell walls. A novel biomimetic method of preparing Au@Ag core-shell NRs was employed, involving deposition of a thin organic polydopamine (PD) primer onto Au NR surfaces, followed by spontaneous electroless silver metallization, and conjugation of antibacterial antibodies and passivating polymers for targeting to gram-negative and gram-positive bacteria. Dramatic cytotoxicity of S. epidermidis and E. coli cells targeted with Au@Ag NRs was observed upon exposure to light as a result of the combined antibacterial effects of plasmonic heating and silver release. The antibacterial effect was much greater than with either plasmonic heating or silver alone, implying a strong therapeutic synergy between cell-targeted plasmonic heating and the associated silver release upon irradiation. Our findings suggest a potential antibacterial use of Au@Ag NRs when coupled with light irradiation, which was not previously described.
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