Ultrasensitive electrochemical immunosensor for procalcitonin with signal enhancement based on zinc nanoparticles functionalized ordered mesoporous carbon-silica nanocomposites
Ultrasensitive electrochemical immunosensor for procalcitonin with signal enhancement based on zinc nanoparticles functionalized ordered mesoporous carbon-silica nanocomposites
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基于锌纳米粒子功能化有序介孔碳-二氧化硅纳米复合材料的信号增强降钙素原超灵敏电化学免疫传感器
DOI：
10.1016/j.snb.2017.11.062
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Wang Lishi
中科院分区：
文献类型：
--
作者：
Fang Yishan;Hu Qiong;Yu Xiongtao;Wang Lishi
基于锌纳米颗粒功能化有序介孔碳-二氧化硅纳米复合材料（OMCSi-Zn），开发了一种新颖且灵敏的降钙素原（PCT）测定策略。该策略结合了有效设计的跟踪标签和简单的免疫传感器。大量的OMCSi-Zn材料作为标记极大地放大了蛋白质电化学检测的检测信号，而石墨烯-壳聚糖作为传感器基质的良好生物相容性为夹心型免疫分析保留了优异的稳定性并加速了电子转移。在没有金属预富集的情况下，负载在有序介孔碳-二氧化硅纳米复合材料（OMCSi）上的锌纳米颗粒可以通过电化学伏安法直接检测到，而免疫反应与不同的伏安峰有密切的关系。该方法对PCT检测表现出高灵敏度和良好的稳定性，线性响应范围从0.05 pg/mL到80 ng/mL，检测限为0.013 pg/mL（S/N = 3）。此外，该方法对临床血清样本进行了准确检测，表明所开发的免疫分析在PCT临床诊断中具有良好的潜在应用。
A novel and sensitive strategy for procalcitonin (PCT) determination was developed based on zinc nanoparticles-functionalized ordered mesoporous carbon-silica nano-composites (OMCSi-Zn). This strategy combined an effectively designed trace tag and a simple immunosensor. The large amount of OMCSi-Zn materials as label greatly amplified the detection signals for the electrochemical detection of proteins, while the good biocompatibility of graphene-chitosan as sensor matrix retained excellent stability and accelerated electron transfer for the sandwich-type immunoassay. Without metal preconcentration, the zinc nanoparticles loading on the ordered mesoporous carbon-silica nano-composites (OMCSi) could directly be detected through electrochemical voltammetries, while the immunoreaction had a close relationship with the distinct voltammetric peaks. The proposed method exhibited high sensitivity and good stability for PCT detection, possessing an improved linear response range from 0.05 pg/mL to 80 ng/mL, with a detection limit of 0.013 pg/mL (S/N = 3). Moreover, the serum samples from clinic were accurately detected with the proposed method, indicating that the developed immunoassay possessed excellent potential application in clinical diagnostics for PCT.
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影响因子：
4.3
作者：
E. Kremmer;K. Meyer;F. Grässer;A. Flatley;Miwako Kösters;P. Luppa;P. Krämer
通讯作者：
E. Kremmer;K. Meyer;F. Grässer;A. Flatley;Miwako Kösters;P. Luppa;P. Krämer
DOI：
10.4103/0972-5229.78214
发表时间：
2011-01
期刊：
Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine
影响因子：
--
作者：
Sudhir U;Venkatachalaiah RK;Kumar TA;Rao MY;Kempegowda P
通讯作者：
Kempegowda P
影响因子：
12.6
作者：
Yishan Fang;Xinjian Huang;Q. Zeng;Lishi Wang
通讯作者：
Yishan Fang;Xinjian Huang;Q. Zeng;Lishi Wang
影响因子：
12.6
作者：
Jiu-Ju Feng;Jingjuan Xu;Hongyuan Chen
通讯作者：
Jiu-Ju Feng;Jingjuan Xu;Hongyuan Chen
影响因子：
12.6
作者：
Lee, S. X.;Lim, H. N.;Huang, N. M.
通讯作者：
Huang, N. M.