LeERF-1, a novel AP2/ERF family gene within the B3 subcluster, is down-regulated by light signals in Lithospermum erythrorhizon.
LeERF-1, a novel AP2/ERF family gene within the B3 subcluster, is down-regulated by light signals in Lithospermum erythrorhizon.
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LeERF-1 是 B3 亚簇内的一种新型 AP2/ERF 家族基因，在紫草中被光信号下调。
DOI：
10.1111/j.1438-8677.2010.00375.x
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Yang, Y.
中科院分区：
文献类型：
--
作者：
Pang, Y.;Yang, R.;Qi, J.;Yang, Y.
我们先前表明，乙烯可能参与由光信号调控的紫草素生物合成过程。在此，我们利用RACE技术从紫草（Lithospermum erythrorhizon）中克隆了一个假定的乙烯响应因子基因LeERF - 1的全长cDNA。
We previously showed that ethylene might be involved in the process of shikonin biosynthesis regulated by light signals. Here, we cloned a full-length cDNA of LeERF-1, a putative ethylene response factor gene, from Lithospermum erythrorhizon using the RACE (rapid amplification of cDNA ends) method. Phylogenetic analysis revealed that LeERF-1 was classified in the B3 subfamily, together with ERF1 and ORA59 of Arabidopsis. Heterologous expression of LeERF-1 in Arabidopsis showed that LeERF-1:eGFP fusion protein was precisely localised to the nucleus, implying that it might function as a transcription factor. Detailed expression analysis with real-time PCR showed that LeERF-1 was significantly down-regulated by white, blue and red light, although the inhibitory effect of red light was relatively weak compared to other light conditions. Tissue-specific expression analysis also indicated that LeERF-1 was dominantly expressed in the roots, which grow in soil in darkness. These patterns are all consistent with the effects of different light signals on regulating formation of shikonin and its derivatives, indicating that LeERF-1 might be a crucial positive regulator, like other B3 subfamily proteins (such as ORCA3 and ORA59), in regulating biosynthesis of secondary metabolites.
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