Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs.
Mouse Heterotopic Cervical Cardiac Transplantation Utilizing Vascular Cuffs.
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DOI：
10.3791/64089
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发表时间：
2022-06-23
期刊：
影响因子：
--
通讯作者：
Kreisel D
中科院分区：
文献类型：
--
作者：
Li W;Shepherd HM;Krupnick AS;Gelman AE;Lavine KJ;Kreisel D
心脏移植的小鼠模型经常用于研究缺血再灌注损伤、移植后的先天性和适应性免疫应答以及免疫调节治疗对移植物排斥的影响。1991年首次描述了小鼠异位颈部心脏移植，采用缝合的心脏移植术，随后修改为包括袖套技术。这一修改提高了成功率，从那时起，有多份报告提出了进一步的技术改进。然而，由于与移植物缝合相关的技术困难，将其转化为更广泛的应用仍然是有限的，这需要精确度以实现套囊的足够长度和口径，以避免可能导致移植物损坏的血管吻合扭曲或过度张力。本方案描述了一种用于在小鼠中进行异位颈部心脏移植的改良技术，其涉及将袖带放置在受体的颈总动脉和供体的肺动脉上，与血流方向对齐。
Murine models of cardiac transplantation are frequently utilized to study ischemia-reperfusion injury, innate and adaptive immune responses after transplantation, and the impact of immunomodulatory therapies on graft rejection. Heterotopic cervical heart transplantation in mice was first described in 1991 using sutured anastomoses and subsequently modified to include cuff techniques. This modification allowed for improved success rates, and since then, there have been multiple reports that have proposed further technical improvements. However, translation into more widespread utilization remains limited due to the technical difficulty associated with graft anastomoses, which requires precision to achieve adequate length and caliber of the cuffs to avoid vascular anastomotic twisting or excessive tension, which can result in damage to the graft. The present protocol describes a modified technique for performing heterotopic cervical cardiac transplantation in mice which involves cuff placement on the recipient’s common carotid artery and the donor’s pulmonary artery in alignment with the direction of the blood flow.
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影响因子：
14.8
作者：
Krupnick, Alexander S.;Lin, Xue;Li, Wenjun;Okazaki, Mikio;Lai, Jiaming;Sugimoto, Seiichiro;Richardson, Steven B.;Kornfeld, Christopher G.;Garbow, Joel R.;Patterson, G. Alexander;Gelman, Andrew E.;Kreisel, Daniel
通讯作者：
Kreisel, Daniel
影响因子：
1.2
作者：
Ratschiller, Thomas;Deutsch, Marcus-Andre;Krane, Markus
通讯作者：
Krane, Markus
影响因子：
6.2
作者：
Tomita, Y;Zhang, QW;Yasui, H
通讯作者：
Yasui, H
DOI：
10.1016/s0022-5223(19)42744-x
发表时间：
1969-01-01
影响因子：
6
作者：
ONO, K;LINDSEY, ES
通讯作者：
LINDSEY, ES
DOI：
10.1007/s10334-014-0466-z
发表时间：
2015-08-01
影响因子：
2.3
作者：
Bovenkamp, Philipp Rene;Brix, Tobias;Hoerr, Verena
通讯作者：
Hoerr, Verena