Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.
Global Profiling of N-Glycoproteins and N-Glycans in the Diatom Phaeodactylum tricornutum.
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硅藻三角褐指藻中 N-糖蛋白和 N-聚糖的整体分析
DOI：
10.3389/fpls.2021.779307
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发表时间：
2021
影响因子：
5.6
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Xie X;Du H;Chen J;Aslam M;Wang W;Chen W;Li P;Du H;Liu X
n -糖基化是所有真核生物中重要的翻译后修饰，但对微藻中的n -糖蛋白和n -聚糖知之甚少。本文采用n -糖蛋白组学和n -糖组学方法揭示了模型三角藻褐指藻的n -糖蛋白和n -聚糖。共鉴定出863种不同的n -糖肽，对应639种n -糖蛋白。这些n -糖蛋白参与了三角草多种重要的代谢途径。12个参与n -糖基化途径的蛋白被鉴定为n -糖蛋白，表明这些蛋白的n -糖基化可能对蛋白质n -糖基化途径很重要。随后，鉴定出59种n -糖蛋白对应的69个n -聚糖，并将其分为高甘露糖型和杂交型n -聚糖。高甘露糖型n-聚糖含有Man-5、Man-7、Man-9和Man-10四种不同的类型，末端有葡萄糖残基。杂化型n-聚糖以末端GlcNAc残基窝藏Man-4。新生蛋白上n -糖基化的鉴定扩大了我们在n -糖蛋白组学尺度上对这种修饰的理解，n -聚糖结构的分析更新了微藻的n -聚糖数据库。本研究结果有助于阐明这些n -糖蛋白的确切功能，为今后设计微藻生产具有功能的人源化生物制药n -糖蛋白，用于临床治疗提供依据。
N-glycosylation is an important posttranslational modification in all eukaryotes, but little is known about the N-glycoproteins and N-glycans in microalgae. Here, N-glycoproteomic and N-glycomic approaches were used to unveil the N-glycoproteins and N-glycans in the model diatom Phaeodactylum tricornutum. In total, 863 different N-glycopeptides corresponding to 639 N-glycoproteins were identified from P. tricornutum. These N-glycoproteins participated in a variety of important metabolic pathways in P. tricornutum. Twelve proteins participating in the N-glycosylation pathway were identified as N-glycoproteins, indicating that the N-glycosylation of these proteins might be important for the protein N-glycosylation pathway. Subsequently, 69 N-glycans corresponding to 59 N-glycoproteins were identified and classified into high mannose and hybrid type N-glycans. High mannose type N-glycans contained four different classes, such as Man-5, Man-7, Man-9, and Man-10 with a terminal glucose residue. Hybrid type N-glycan harbored Man-4 with a terminal GlcNAc residue. The identification of N-glycosylation on nascent proteins expanded our understanding of this modification at a N-glycoproteomic scale, the analysis of N-glycan structures updated the N-glycan database in microalgae. The results obtained from this study facilitate the elucidation of the precise function of these N-glycoproteins and are beneficial for future designing the microalga to produce the functional humanized biopharmaceutical N-glycoproteins for the clinical therapeutics.
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影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
影响因子：
16.6
作者：
Daboussi, Fayza;Leduc, Sophie;Duchateau, Philippe
通讯作者：
Duchateau, Philippe
影响因子：
4.8
作者：
Levy-Ontman, Oshrat;Arad, Shoshana (Malis);Tekoah, Yoram
通讯作者：
Tekoah, Yoram
DOI：
10.1016/j.algal.2016.10.018
发表时间：
2016-12-01
影响因子：
5.1
作者：
Liu, Xiaojuan;Hempel, Franziska;Zauner, Stefan
通讯作者：
Zauner, Stefan
影响因子：
5.8
作者：
Cheng, Alice;Grant, Charles E.;Bailey, Timothy L.
通讯作者：
Bailey, Timothy L.