In Vitro and In Vivo Gastrointestinal Survival of Non-Encapsulated and Microencapsulated Salmonella Bacteriophages: Implications for Bacteriophage Therapy in Poultry.
In Vitro and In Vivo Gastrointestinal Survival of Non-Encapsulated and Microencapsulated Salmonella Bacteriophages: Implications for Bacteriophage Therapy in Poultry.
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体外和体内胃肠道生存的非封闭和微囊泡沙门氏菌噬菌体的生存：对家禽中噬菌体治疗的影响。
DOI：
10.3390/ph14050434
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发表时间：
2021-05-06
期刊：
影响因子：
--
通讯作者：
Sevilla-Navarro S
中科院分区：
文献类型：
--
作者：
Lorenzo-Rebenaque L;Malik DJ;Catalá-Gregori P;Marin C;Sevilla-Navarro S
噬菌体的治疗作用被认为是控制沙门氏菌的一种可行方法。在口服剂型中微胶囊化噬菌体可以保护噬菌体免受鸡胃肠道的固有挑战。因此，本研究的主要目的是评估沙门氏菌BPFGS011(非囊化和微囊化)在体外和体内条件下口服给药1日龄雏鸡后在胃肠道的存活情况。为此，采用喷雾干燥工艺将噬菌体FGS011包裹在两种不同的pH响应型配方中，聚合物分别为Eudradit®L100和Eudradit®S100。在体外条件下，包裹在两种制剂中的噬菌体能够在腺胃-肌囊中存活，而游离噬菌体则不能。此外，在聚合物Eudradit®S100中配制的噬菌体更适合将噬菌体输送到鸡的盲肠。在体内试验中，无论是游离噬菌体还是胶囊噬菌体给雏鸡，胃肠道内的噬菌体浓度都没有统计学上的差异。这表明雏鸡的腺胃/肌胃的pH值不足以引起不同的噬菌体滴度下降，从而允许游离噬菌体在体内存活。
The therapeutic use of bacteriophages is recognized as a viable method to control Salmonella. Microencapsulation of phages in oral dosage forms may protect phages from inherent challenges of the gastrointestinal tract in chickens. Therefore, the main objective of this study was to assess the survival of Salmonella BP FGS011 (non-encapsulated and microencapsulated) through the gastrointestinal tract under in vitro as well as in vivo conditions after oral administration to 1-day-old chicks. To this end, the phage FGS011 was encapsulated in two different pH-responsive formulations with polymers Eudragit® L100, and Eudragit® S100 using the process of spray drying. Phages encapsulated in either of the two formulations were able to survive exposure to the proventriculus-gizzard in vitro conditions whereas free phages did not. Moreover, phages formulated in polymer Eudragit® S100 would be better suited to deliver phage to the caeca in chickens. In the in vivo assay, no statistically significant differences were observed in the phage concentrations across the gastrointestinal tract for either the free phage or the encapsulated phage given to chicks. This suggested that the pH of the proventriculus/gizzard in young chicks is not sufficiently acidic to cause differential phage titre reductions, thereby allowing free phage survival in vivo.
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影响因子：
5.2
作者：
Bull JJ;Gill JJ
通讯作者：
Gill JJ
肠道沙门氏菌：血清射手之间的生存，定殖和毒力差异。
影响因子：
--
作者：
Andino A;Hanning I
通讯作者：
Hanning I
影响因子：
3
作者：
Kim, Shukho;Kim, Sung-Hun;Kim, Jungmin
通讯作者：
Kim, Jungmin
影响因子：
3.7
作者：
Berry, Elaine D.;Wells, James E.
通讯作者：
Wells, James E.
影响因子：
4.4
作者：
Adhikari, P. A.;Cosby, D. E.;Kim, W. K.
通讯作者：
Kim, W. K.