DNA Microarray-Based Global Gene Expression Profiling in Human Amniotic Epithelial Cells Predicts the Potential of Microalgae-Derived Squalene for the Nervous System and Metabolic Health.
DNA Microarray-Based Global Gene Expression Profiling in Human Amniotic Epithelial Cells Predicts the Potential of Microalgae-Derived Squalene for the Nervous System and Metabolic Health.
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人类羊膜上皮细胞中基于DNA微阵列的全球基因表达谱预测微藻衍生的角鲨烯对神经系统和代谢健康的潜力。
DOI：
10.3390/biomedicines10010048
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发表时间：
2021-12-27
期刊：
影响因子：
4.7
通讯作者：
Isoda H
中科院分区：
文献类型：
--
作者：
Ferdousi F;Furuya K;Sasaki K;Zheng YW;Oda T;Isoda H
近年来，围产期干细胞，如人羊膜上皮细胞（hAECs），作为一种新的干细胞为基础的高通量药物筛选的工具，吸引了越来越多的兴趣。在本研究中，我们研究了角鲨烯（SQ）的乙醇提取物（99.5%）的微藻橙壶菌（EEA-SQ）在hAEC的生物活性，使用全基因组DNA微阵列分析。组织富集分析显示，脑是EEA-SQ处理的hAEC和对照hAEC之间差异表达基因（DEG）最显著富集的组织。进一步的基因集富集分析和组织特异性功能分析揭示了与神经系统发育、神经发生和神经递质调节相关的生物学功能。一些脂肪组织特异性基因和功能也得到了富集。基因-疾病关联分析显示，神经系统、代谢和免疫相关疾病较为丰富。总之，我们的研究表明微藻衍生SQ的潜在健康益处，我们将进一步鼓励研究EEA-SQ及其衍生物作为神经系统和代谢相关疾病的潜在治疗药物。
In recent years, perinatal stem cells, such as human amniotic epithelial cells (hAECs), have attracted increasing interest as a novel tool of stem cell-based high-throughput drug screening. In the present study, we investigated the bioactivities of squalene (SQ) derived from ethanol extract (99.5%) of a microalgae Aurantiochytrium Sp. (EEA-SQ) in hAECs using whole-genome DNA microarray analysis. Tissue enrichment analysis showed that the brain was the most significantly enriched tissue by the differentially expressed genes (DEGs) between EEA-SQ-treated and control hAECs. Further gene set enrichment analysis and tissue-specific functional analysis revealed biological functions related to nervous system development, neurogenesis, and neurotransmitter modulation. Several adipose tissue-specific genes and functions were also enriched. Gene-disease association analysis showed nervous system-, metabolic-, and immune-related diseases were enriched. Altogether, our study suggests the potential health benefits of microalgae-derived SQ and we would further encourage investigation in EEA-SQ and its derivatives as potential therapeutics for nervous system- and metabolism-related diseases.
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影响因子：
14.9
作者：
Dougherty JD;Schmidt EF;Nakajima M;Heintz N
通讯作者：
Heintz N
DOI：
10.1186/s12964-020-00697-5
发表时间：
2021-02-24
期刊：
Cell communication and signaling : CCS
影响因子：
--
作者：
Bejaoui M;Ferdousi F;Zheng YW;Oda T;Isoda H
通讯作者：
Isoda H
影响因子：
4
作者：
Kowiański P;Lietzau G;Czuba E;Waśkow M;Steliga A;Moryś J
通讯作者：
Moryś J
影响因子：
5.5
作者：
Ganbold M;Ferdousi F;Arimura T;Tominaga K;Isoda H
通讯作者：
Isoda H
影响因子：
14.9
作者：
Davis AP;Grondin CJ;Johnson RJ;Sciaky D;Wiegers J;Wiegers TC;Mattingly CJ
通讯作者：
Mattingly CJ