Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model.
Bacteroides fragilis strain ZY-312 promotes intestinal barrier integrity via upregulating the STAT3 pathway in a radiation-induced intestinal injury mouse model.
复制标题
脆弱类杆菌菌株ZY-312通过上调STAT3途径在辐射诱导的肠道损伤小鼠模型中促进肠道屏障的完整性。
DOI：
10.3389/fnut.2022.1063699
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发表时间：
2022
影响因子：
5
通讯作者：
Zhi, Fachao
中科院分区：
文献类型：
--
作者：
Zhou, Qian;Shen, Binhai;Huang, Ruo;Liu, Hongbin;Zhang, Wendi;Song, Mengyao;Liu, Ke;Lin, Xinlong;Chen, Shuze;Liu, Yangyang;Wang, Ye;Zhi, Fachao
关键词：
放射性肠损伤的特点是肠屏障功能受损。然而，益生菌对辐射诱导的肠损伤小鼠模型中肠上皮屏障修复的治疗作用仍不清楚。此前，我们从健康婴儿的粪便中分离出一株脆弱拟杆菌，并将其命名为B。fragilis菌株ZY-312（B. fragilis）。在这项研究中，我们发现，B。fragilis可改善小鼠辐射诱导的肠损伤，表现为体重减轻、肠长度缩短和肠上皮细胞（IEC）脱落减少。此外，我们发现B.通过在Stat 3 △IEC小鼠（肠上皮细胞中的STAT 3缺陷）中的实验验证，fragilis通过上调STAT 3信号通路促进IEC增殖、干细胞再生、粘液分泌和紧密连接完整性。因此，B.在辐射诱导的肠损伤中，脆弱性与IEC增殖、干细胞再生、杯状细胞分泌和通过激活STAT 3信号通路的紧密连接修复有关。此外，观察了B. fragilis的研究，以提供新的见解，其应用作为一个功能和临床药物放射治疗后的放射性肠损伤。
Radiation-induced intestinal injury is characterized by intestinal barrier impairment. However, the therapeutic effects of probiotics for intestinal epithelial barrier repair in a mouse model of radiation-induced intestinal injury remain unclear. Previously, we isolated a strain of Bacteroides fragilis from the feces of a healthy infant and named it as B. fragilis strain ZY-312 (B. fragilis). In this study, we showed that B. fragilis can ameliorate radiation-induced intestinal injury in mice, manifested by decreased weight loss, intestinal length shortening, and intestinal epithelial cell (IEC) shedding. Moreover, we found that B. fragilis promoted IEC proliferation, stem cell regeneration, mucus secretion, and tight junction integrity by upregulating the STAT3 signaling pathway, through an experimental verification in Stat3△IEC mice (STAT3 defects in intestinal epithelial cells). Thus, the underlying protective mechanism of B. fragilis in radiation-induced intestinal injury is related to IEC proliferation, stem cell regeneration, goblet cell secretion, and tight junction repair via activation of the STAT3 signaling pathway. In addition, the therapeutic effects of B. fragilis were studied to provide new insights into its application as a functional and clinical drug for radiation-induced intestinal injury after radiotherapy.
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DOI：
10.1038/nri.2016.88
发表时间：
2016-10
期刊：
Nature reviews. Immunology
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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影响因子：
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作者：
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