Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly.
Fibrogranular materials function as organizers to ensure the fidelity of multiciliary assembly.
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纤维颗粒材料充当组织者，确保多纤毛组装的保真度
DOI：
10.1038/s41467-021-21506-8
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发表时间：
2021-02-24
影响因子：
16.6
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Zhao H;Chen Q;Li F;Cui L;Xie L;Huang Q;Liang X;Zhou J;Yan X;Zhu X
Multicilia是一种精密的移动机器，人们对它们是如何准确组装的知之甚少。在这里，我们展示了纤维颗粒材料(FGM)，多纤毛细胞特有的电子致密颗粒的大阵列，对于它们的超微结构保真度是必不可少的。Pcm1形成颗粒单元，进一步形成广泛分布的功能梯度材料，在球形功能梯度材料核心中含量丰富。FGM核心选择性地集中多种重要的中心粒相关蛋白作为客户，包括Cep131，它专门装饰纤毛中心对(CP)微管的足部区域。FGM还紧密接触后小体-原着丝点复合体。通过Pcm1 RNAi破坏小鼠细胞中的FGM，显著地解除对FGM客户的中心粒靶向的调控，延长CP-Foot，并改变后体的大小、数量和分布。虽然多纤毛的产生数量正确，但它们表现出异常的超微结构和运动性。我们的结果表明，FGM组织了后体和中心粒相关的蛋白，以促进基本体和多纤毛轴丝的忠实组装。
Multicilia are delicate motile machineries, and how they are accurately assembled is poorly understood. Here, we show that fibrogranular materials (FGMs), large arrays of electron-dense granules specific to multiciliated cells, are essential for their ultrastructural fidelity. Pcm1 forms the granular units that further network into widespread FGMs, which are abundant in spherical FGM cores. FGM cores selectively concentrate multiple important centriole-related proteins as clients, including Cep131 that specifically decorates a foot region of ciliary central pair (CP) microtubules. FGMs also tightly contact deuterosome-procentriole complexes. Disruption of FGMs in mouse cells undergoing multiciliogenesis by Pcm1 RNAi markedly deregulates centriolar targeting of FGM clients, elongates CP-foot, and alters deuterosome size, number, and distribution. Although the multicilia are produced in correct numbers, they display abnormal ultrastructure and motility. Our results suggest that FGMs organize deuterosomes and centriole-related proteins to facilitate the faithful assembly of basal bodies and multiciliary axonemes.
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影响因子：
64.8
作者：
Al Jord, Adel;Lemaitre, Anne-Iris;Meunier, Alice
通讯作者：
Meunier, Alice
影响因子：
56.9
作者：
Al Jord, Adel;Shihavuddin, Asm;Meunier, Alice
通讯作者：
Meunier, Alice
影响因子：
3.3
作者：
Fong, Ka-Wing;Choi, Yuk-Kwan;Qi, Robert Z.
通讯作者：
Qi, Robert Z.
DOI：
10.1016/j.cub.2014.08.047
发表时间：
2014-10-06
期刊：
Current biology : CB
影响因子：
--
作者：
Brooks ER;Wallingford JB
通讯作者：
Wallingford JB
影响因子：
16.6
作者：
Mahuzier A;Shihavuddin A;Fournier C;Lansade P;Faucourt M;Menezes N;Meunier A;Garfa-Traoré M;Carlier MF;Voituriez R;Genovesio A;Spassky N;Delgehyr N
通讯作者：
Delgehyr N