Ependymal cilia beating induces an actin network to protect centrioles against shear stress.
Ependymal cilia beating induces an actin network to protect centrioles against shear stress.
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DOI：
10.1038/s41467-018-04676-w
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发表时间：
2018-06-11
影响因子：
16.6
通讯作者：
Delgehyr N
中科院分区：
文献类型：
--
作者：
Mahuzier A;Shihavuddin A;Fournier C;Lansade P;Faucourt M;Menezes N;Meunier A;Garfa-Traoré M;Carlier MF;Voituriez R;Genovesio A;Spassky N;Delgehyr N
多纤毛室管膜细胞排列在所有的脑腔中。它们的运动纤毛的跳动有助于脑脊髓液的流动，这是大脑稳态和功能所必需的。从中心粒成核的运动纤毛一旦形成就持续存在，并承受外部流体流动和自身纤毛跳动产生的力。在这里，我们表明，一个密集的肌动蛋白网络周围的中心粒诱导纤毛跳动，如所示的解体的肌动蛋白网络损害纤毛运动。此外，肌动蛋白网络的破坏，或特别是顶端肌动蛋白网络的破坏，导致运动纤毛和它们的中心粒从室管膜细胞的顶端表面脱离。总之，纤毛跳动控制着中心粒周围的顶端肌动蛋白网络;肌动蛋白网络的机械阻力反过来又有助于中心粒的稳定。室管膜纤毛搏动有助于脑室内脑脊液的流动，这些纤毛抵抗流动力。在这里，作者表明，一个致密的肌动蛋白网络周围的中心粒组装诱导纤毛跳动，以保护中心粒免受纤毛运动产生的剪切应力。
Multiciliated ependymal cells line all brain cavities. The beating of their motile cilia contributes to the flow of cerebrospinal fluid, which is required for brain homoeostasis and functions. Motile cilia, nucleated from centrioles, persist once formed and withstand the forces produced by the external fluid flow and by their own cilia beating. Here, we show that a dense actin network around the centrioles is induced by cilia beating, as shown by the disorganisation of the actin network upon impairment of cilia motility. Moreover, disruption of the actin network, or specifically of the apical actin network, causes motile cilia and their centrioles to detach from the apical surface of ependymal cell. In conclusion, cilia beating controls the apical actin network around centrioles; the mechanical resistance of this actin network contributes, in turn, to centriole stability. Ependymal ciliary beating contributes to the flow of cerebrospinal fluid in the brain ventricles and these cilia resist the flow forces. Here the authors show that the assembly of a dense actin network around the centrioles is induced by cilia beating to protect centrioles against the shear stress generated by ciliary motility.
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影响因子：
2.9
作者：
Grega-Larson, Nathan E.;Crawley, Scott W.;Tyska, Matthew J.
通讯作者：
Tyska, Matthew J.
DOI：
10.1083/jcb.201604135
发表时间：
2016-11-21
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bayless BA;Galati DF;Junker AD;Backer CB;Gaertig J;Pearson CG
通讯作者：
Pearson CG
影响因子：
11.4
作者：
BAILLY, E;DOREE, M;BORNENS, M
通讯作者：
BORNENS, M
影响因子：
2.7
作者：
Carroll, EA;Gerrelli, D;Klingensmith, J
通讯作者：
Klingensmith, J
影响因子：
64.5
作者：
Ahuja, Rashmi;Pinyol, Roser;Qualmann, Britta
通讯作者：
Qualmann, Britta