Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems.
Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems.
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DOI：
10.1016/j.xpro.2021.100733
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发表时间：
2021-09-17
期刊：
影响因子：
--
通讯作者：
Bi E
中科院分区：
文献类型：
--
作者：
Okada H;MacTaggart B;Bi E
基于显微镜的蛋白质积累分析在一个给定的亚细胞位置在真实的时间提供了宝贵的见解蛋白质的功能在一个特定的过程。在这里，我们描述了一个详细的协议，用于确定在芽殖酵母酿酒酵母和裂殖酵母粟酒裂殖酵母分裂位点的蛋白质积累动力学。该方案可适用于分析任何过程中涉及的任何蛋白质，只要该蛋白质定位于细胞的离散区域。有关本方案使用和执行的完整详细信息，请参见和。酵母系统中活细胞成像的关键因素蛋白质定位动力学分析的详细协议肌球蛋白-II在分裂位点积累的动力学分析揭示生物学机制的动力学分析的局限性基于显微镜的蛋白质积累分析在给定的亚细胞位置在真实的时间提供了宝贵的见解蛋白质的功能在一个特定的过程。在这里，我们描述了一个详细的协议，用于确定在芽殖酵母酿酒酵母和裂殖酵母粟酒裂殖酵母分裂位点的蛋白质积累动力学。该方案可适用于分析任何过程中涉及的任何蛋白质，只要该蛋白质定位于细胞的离散区域。
Microscopy-based analysis of protein accumulation at a given subcellular location in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe. This protocol can be adapted for the analysis of any protein involved in any process as long as the protein is localized to a discrete region of the cell. For complete details on the use and execution of this protocol, please refer to and. Critical factors for live-cell imaging in yeast systems Detailed protocol for analyzing protein localization kinetics Kinetic analysis of myosin-II accumulation at the division site Limitations of kinetic analysis in uncovering biological mechanisms Microscopy-based analysis of protein accumulation at a given subcellular location in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding yeast Saccharomyces cerevisiae and fission yeast Schizosaccharomyces pombe. This protocol can be adapted for the analysis of any protein involved in any process as long as the protein is localized to a discrete region of the cell.
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DOI：
10.1073/pnas.92.7.2984
发表时间：
1995-03-28
影响因子：
11.1
作者：
BHATTACHARYA, S;CHEN, L;POWERS, S
通讯作者：
POWERS, S
影响因子：
14.9
作者：
Dubreuil B;Sass E;Nadav Y;Heidenreich M;Georgeson JM;Weill U;Duan Y;Meurer M;Schuldiner M;Knop M;Levy ED
通讯作者：
Levy ED
影响因子：
--
作者：
Babic J;Griscom L;Cramer J;Coudreuse D
通讯作者：
Coudreuse D
影响因子：
64.5
作者：
Colman-Lerner, A;Chin, TE;Brent, R
通讯作者：
Brent, R
影响因子：
--
作者：
Torres-Garcia S;Di Pompeo L;Eivers L;Gaborieau B;White SA;Pidoux AL;Kanigowska P;Yaseen I;Cai Y;Allshire RC
通讯作者：
Allshire RC