Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair.
Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair.
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DOI：
10.1016/j.burns.2015.06.011
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发表时间：
2015-12
期刊：
影响因子：
--
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Mahjour SB;Fu X;Yang X;Fong J;Sefat F;Wang H
在临床可接受的时间窗口内创建功能性皮肤替代品对于及时修复和处理大面积烧伤等大型伤口至关重要。这项研究的目的是探索通过自下而上的纳米纤维细胞组装方法制造皮肤替代物并将其用于裸鼠全层伤口修复的可能性。采用L-b-L的方法，将人皮肤原代细胞(成纤维细胞和角质形成细胞)与电纺聚己内酯/胶原(3：1 w/w，8%w/v)纳米纤维快速组装成三维结构，成纤维细胞和角质形成细胞分别位于底部和上部。培养后，构建物发育为类皮肤结构，表达基础角质形成细胞标志并沉积新的基质，同时表现出良好的机械强度(14d时高达4.0 Mpa)。用不同的移植物(无细胞纳米纤维网、真皮替代物、皮肤替代物和自体移植物)治疗裸鼠背部的全层创面发现，14天培养的皮肤替代物促进了伤口的快速愈合，与自体移植物相当，具有完全的上皮化。综上所述，L-b-L将人皮肤细胞和仿生纳米纤维组装在一起，可以形成类皮肤替代物，对裸鼠急性全层创面的愈合是有效的。
Creation of functional skin substitutes within a clinically acceptable time window is essential for timely repair and management of large wounds such as extensive burns. The aim of this study was to investigate the possibility of fabricating skin substitutes via a bottom-up nanofiber-enabled cell assembly approach and using such substitutes for full-thickness wound repair in nude mice. Following a layer-by-layer (L-b-L) manner, human primary skin cells (fibroblasts and keratinocytes) were rapidly assembled together with electrospun polycaprolactone (PCL)/collagen (3:1 w/w, 8% w/v) nanofibers into 3D constructs, in which fibroblasts and keratinocytes were located in the bottom and upper portion respectively. Following culture, the constructs developed into a skin-like structure with expression of basal keratinocyte markers and deposition of new matrix while exhibiting good mechanical strength (as high as 4.0 MPa by 14 days). Treatment of the full-thickness wounds created on the back of nude mice with various grafts (acellular nanofiber meshes, dermal substitutes, skin substitutes and autografts) revealed that 14-day-cultured skin substitutes facilitated a rapid wound closure with complete epithelialization comparable to autografts. Taken together, skin-like substitutes can be formed by L-b-L assembling human skin cells and biomimetic nanofibers and they are effective to heal acute full-thickness wounds in nude mice.
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影响因子：
14
作者：
Fu X;Xu M;Liu J;Qi Y;Li S;Wang H
通讯作者：
Wang H
影响因子：
14.5
作者：
Leopold PL;Vincent J;Wang H
通讯作者：
Wang H
壳聚糖/胶原组装的仿生LBL结构纳米纤维基质促进伤口愈合
影响因子：
14
作者：
Huang, Rong;Li, Wangzhou;Li, Xueyong
通讯作者：
Li, Xueyong
影响因子：
14
作者：
Huang, Chengyang;Fu, Xiaoling;Liu, Jie;Qi, Yanmei;Li, Shaohua;Wang, Hongjun
通讯作者：
Wang, Hongjun
DOI：
10.1016/j.jmbbm.2011.08.016
发表时间：
2012-01-01
影响因子：
3.9
作者：
Annaidh, Aisling Ni;Bruyere, Karine;Ottenio, Melanie
通讯作者：
Ottenio, Melanie