Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice.
Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice.
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DOI：
10.1083/jcb.201011110
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发表时间：
2011-04-04
期刊：
影响因子：
--
通讯作者：
Hirose T
中科院分区：
文献类型：
--
作者：
Nakagawa S;Naganuma T;Shioi G;Hirose T
小鼠NEAT1是在部分细胞中形成副斑点所必需的，但对动物的健康和生存能力并不是必需的。高等生物的核结构良好，有多个不同的核室或核体。Paraspeckles是最近发现的哺乳动物特有的核小体，广泛存在于大多数体外培养的细胞中。为了研究副核蛋白的生理作用，我们研究了两个长的非编码RNA NEAT1_1和NEAT1_2的体内表达模式，这两个RNA对于核体的结构完整性是必不可少的。出乎意料的是，这些基因只在成年小鼠组织中的特定细胞亚群中强表达，并且只有在高表达NEAT1_2的细胞中才能观察到显著的副斑点形成。为了进一步研究副斑点的细胞功能，我们通过基因打靶建立了一个缺失NEAT1的动物模型。这些基因敲除的小鼠在实验室的生长条件下是存活和可生育的，除了副突起消失外，没有表现出明显的表型。我们认为，副核小体是非必需的、亚群特有的核体，形成于特定的环境触发因素。
Mouse NEAT1 is required for paraspeckle formation in a subset of cells but is not essential for animal health and viability. Nuclei of higher organisms are well structured and have multiple, distinct nuclear compartments or nuclear bodies. Paraspeckles are recently identified mammal-specific nuclear bodies ubiquitously found in most cells cultured in vitro. To investigate the physiological role of paraspeckles, we examined the in vivo expression patterns of two long noncoding RNAs, NEAT1_1 and NEAT1_2, which are essential for the architectural integrity of nuclear bodies. Unexpectedly, these genes were only strongly expressed in a particular subpopulation of cells in adult mouse tissues, and prominent paraspeckle formation was observed only in the cells highly expressing NEAT1_2. To further investigate the cellular functions of paraspeckles, we created an animal model lacking NEAT1 by gene targeting. These knockout mice were viable and fertile under laboratory growth conditions, showing no apparent phenotypes except for the disappearance of paraspeckles. We propose that paraspeckles are nonessential, subpopulation-specific nuclear bodies formed secondary to particular environmental triggers.
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