Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets.
Response of Gut Microbiota to Dietary Fiber and Metabolic Interaction With SCFAs in Piglets.
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DOI：
10.3389/fmicb.2018.02344
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发表时间：
2018
影响因子：
5.2
通讯作者：
Shi Y
中科院分区：
文献类型：
--
作者：
Liu B;Wang W;Zhu X;Sun X;Xiao J;Li D;Cui Y;Wang C;Shi Y
日粮纤维（DF）被认为可以调节仔猪肠道菌群的多样性，缓解仔猪断奶应激。本研究旨在探讨DF对仔猪生长性能和肠道菌群组成的影响，以及短链脂肪酸（SCFAs）与肠道菌群的相互作用。将840头仔猪随机分为对照组（CG）、苜蓿草粉组（AG）和商品浓缩纤维组（OG），进行为期30天的饲养试验。采用16 S rRNA基因扩增子测序法测定肠道粘膜和粪便样品中细菌群落多样性。纤维处理对仔猪的生长性能和SCFAs的代谢有积极的影响，特别是与对照组相比，AG组腹泻率显著降低，盲肠中丙酸（PA）含量显著增加。AG组空肠菌群的Shannon指数高于CG组。在属的水平上，与对照组相比，十二指肠中AG和OG类芽孢杆菌的相对丰度较高，空肠中芽孢杆菌、海洋芽孢杆菌、类芽孢杆菌、乳球菌、肠球菌和类芽孢杆菌的相对丰度较高，而支原体的相对丰度较低;盲肠中幽门螺杆菌的相对丰度AG和OG也较低，粪杆菌的相对丰度OG高于CG和AG。斯皮尔曼相关分析表明，假丁酸弧菌与乙酸、PA和丁酸（BA）呈正相关，拟杆菌和厌氧干杆菌与PA和BA呈负相关。此外，不同肠段之间的微生物群分析显示近端和远端肠之间的微生物群存在明显差异。近段细菌主要为厚壁菌门，远段细菌主要为拟杆菌门和厚壁菌门。总体而言，这些发现表明DF治疗可以降低仔猪的腹泻率，并对肠道健康具有有益的影响，这可能归因于DF诱导的肠道微生物群的改变以及肠道微生物群与SCFA的相互作用。
Dietary fiber (DF) is increasingly thought to regulate diversity of piglet gut microbiota to alleviate weaning stress in piglets. This study was conducted to investigate the effects of DF on growth performance of piglets and composition of their gut microbiota, as well as the interaction between gut microbiota and short-chain fatty acids (SCFAs) in piglets. A total of 840 piglets were allocated to three dietary treatments consisting of a control group (CG), an alfalfa meal group (AG), and a commodity concentrated fiber group (OG) in a 30-day feeding trial. Gut mucosa and feces samples were used to determine bacterial community diversity by 16S rRNA gene amplicon sequencing. Fiber treatment had a positive effect on growth performance and metabolism of SCFAs in piglets, in particular, compared with CG, the diarrhea rate was significantly decreased, and the content of propionic acid (PA) in the cecum was markedly increased in AG. The Shannon indices of the jejunum microbiota in AG were higher than CG. At the genus level, compared to CG, in the duodenum, the relative abundance of Paenibacillus in AG and OG was higher; in the jejunum, the relative abundances of Bacillus, Oceanobacillus, Paenibacillus, Lactococcus, Enterococcus, and Exiguobacterium were higher, whereas the relative abundance of Mycoplasma was lower in AG; in the cecum, there was also lower relative abundance of Helicobacter in AG and OG, and furthermore, the relative abundance of Faecalibacterium in OG was higher than in CG and AG. Spearman correlation analysis showed that Pseudobutyrivibrio was positively correlated with acetic acid, PA, and butyric acid (BA), while Bacteroides and Anaerotruncus were negatively correlated with PA and BA. In addition, microbiota analyses among different intestine segments showed distinct differences in microbiota between the proximal and distal intestines. Bacteria in the proximal segments were mainly Firmicutes, while bacteria in the distal segments were mainly Bacteroidetes and Firmicutes. Overall, these findings suggested that DF treatment could reduce the diarrhea rate of piglets and had beneficial effects on gut health, which might be attributed to the alteration in gut microbiota induced by DF and the interaction of the gut microbiota with SCFAs.
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影响因子：
3.7
作者：
Heinritz SN;Weiss E;Eklund M;Aumiller T;Louis S;Rings A;Messner S;Camarinha-Silva A;Seifert J;Bischoff SC;Mosenthin R
通讯作者：
Mosenthin R
影响因子：
3.7
作者：
Hu Y;Dun Y;Li S;Zhang D;Peng N;Zhao S;Liang Y
通讯作者：
Liang Y
影响因子：
3.3
作者：
Castillo, Marisol;Martin-Orue, Susana M.;Gasa, Josep
通讯作者：
Gasa, Josep
影响因子：
3.7
作者：
Fouhy F;Deane J;Rea MC;O'Sullivan Ó;Ross RP;O'Callaghan G;Plant BJ;Stanton C
通讯作者：
Stanton C
影响因子：
6.3
作者：
Alfa, Michelle J.;Strang, David;Lix, Lisa M.
通讯作者：
Lix, Lisa M.