Effect of Chemical Additives on Bacillus thuringiensis (Bacillales: Bacillaceae) Against Plutella xylostella (Lepidoptera: Pyralidae)

Effect of Chemical Additives on Bacillus thuringiensis (Bacillales: Bacillaceae) Against Plutella xylostella (Lepidoptera: Pyralidae)
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化学添加剂对苏云金芽孢杆菌(芽孢杆菌目:芽孢杆菌科)对抗小菜蛾(鳞翅目:螟虫科)的影响

DOI:
10.1603/ec12288
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发表时间:
2013-06
影响因子:
2.2
通讯作者:
Guan, X.
Guan, X.
中科院分区:
农林科学2区
文献类型:
--
作者:
Zhang, L.;Qiu, S.;Huang, T.;Huang, Z.;Xu, L.;Wu, C.;Gelbic, I.;Guan, X.

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摘要为了研究化学添加剂对苏云金芽孢杆菌(Berliner)防治小菜蛾的作用,选择并测试无机盐、含氮化合物、蛋白质增溶剂和有机酸。所选择的材料成本低且对环境安全。结果表明,多种无机盐能提高B的活性。苏云金杆菌的毒力在1.31- 3.08倍之间。这些包括乙酸钙、氯化钙、氢氧化钙、硫酸钙、碳酸钙、碳酸钠、乙酸钠、氢氧化钾、碳酸钾、乙酸钾、氯化镁、硫酸镁和硫酸锌。含氮化合物,包括蛋白胨、硝酸钠和硝酸铵,可以增强B的活性。苏云金芽孢杆菌(Thuringiensisensis)的毒力分别为1.62、1.32和1.37倍。在蛋白增溶剂中,EDTA、尿素、巯基乙醇和磷酸氢二钾提高了B的活性。苏云金杆菌1.62 ~ 2.34倍。在有机酸中,马来酸和柠檬酸分别提高活性1.45倍和1.55倍。同时,苯甲酸钠和间苯二酚分别导致1.74和1.44倍的活性增益。使用适当的添加剂不仅可以降低生物杀虫剂的田间应用成本,而且可以提高B的药效。苏云金杆菌
ABSTRACT To examine the effect of chemical additives on Bacillus thuringiensis (Berliner) against Plutella xylostella (L.), inorganic salts, nitrogenous compounds, protein solubilizing agents, and organic acids were selected and tested. The chosen materials are low in cost and environmentally safe. Results show that many inorganic salts can increase the activity of B. thuringiensis in a range of 1.31-to 3.08-fold. These include calcium acetate, calcium chloride, calcium hydroxide, calcium sulfate, calcium carbonate, sodium carbonate, sodium acetate, potassium hydroxide, potassium carbonate, potassium acetate, magnesium chloride, magnesium sulfate, and zinc sulfate. Nitrogenous compounds, including peptone, sodium nitrate, and ammonium nitrate, can enhance the activity of B. thuringiensis 1.62-, 1.32-, and 1.37-fold, respectively. Among the protein solubilizing agents, EDTA, urea, mercaptoethanol and dipotassium hydrogen phosphate increased the activity of B. thuringiensis 1.62- to 2.34-fold. Among the organic acids, maleic and citric acids boosted the activity 1.45- and 1.55-fold, respectively. Meanwhile, sodium benzoate and resorcinol led to 1.74- and 1.44-fold activity gains, respectively. Use of appropriate additives could provide great benefit not only in reducing the costs for field applications of biological insecticides but also by boosting the efficacy of B. thuringiensis.
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发表时间: 2004
影响因子: 2.3
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