Telocytes in human skin--are they involved in skin regeneration?
Telocytes in human skin--are they involved in skin regeneration?
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DOI：
10.1111/j.1582-4934.2012.01580.x
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发表时间：
2012-07
影响因子：
5.3
通讯作者：
Simionescu O
中科院分区：
文献类型：
--
作者：
Ceafalan L;Gherghiceanu M;Popescu LM;Simionescu O
远端细胞（TCs）是一种特殊的间质细胞类型，最近在多种哺乳动物器官中被发现（）。这些tc在形态学上由一个很小的细胞体和极长（几十到几百μm）的细长延伸（直径小于100 nm，低于光学显微镜的分辨能力）来识别。在这里，我们用电子显微镜和免疫荧光证明了TCs存在于人类真皮中。特别是在网状真皮层、血管周围、毛囊周围鞘、玻璃膜外和皮脂腺周围、立毛肌以及汗腺的分泌和排泄部分均发现了TCs。免疫荧光筛选和激光扫描共聚焦显微镜显示真皮TCs的两个亚群；一个表达c-kit/CD117，另一个表达CD34。两个亚群的vimentin均呈阳性。TCs通过同质细胞连接相互连接，形成间质三维网络。我们还在毛囊的凸起区域发现了与干细胞相连的TCs。此外，TCs与干细胞（未分化的细胞群）建立了非典型的异细胞连接。鉴于过敏性皮肤病变的频率，我们想强调的是，在tc和肥大细胞之间经常观察到紧密的平面连接。总之，基于TC的分布和细胞间连接，我们的研究结果表明TC可能参与皮肤稳态、皮肤重塑、皮肤再生和皮肤修复。
Telocytes (TCs), a particular interstitial cell type, have been recently described in a wide variety of mammalian organs (). The TCs are identified morphologically by a small cell body and extremely long (tens to hundreds of μm), thin prolongations (less than 100 nm in diameter, below the resolving power of light microscopy) called telopodes. Here, we demonstrated with electron microscopy and immunofluorescence that TCs were present in human dermis. In particular, TCs were found in the reticular dermis, around blood vessels, in the perifollicular sheath, outside the glassy membrane and surrounding sebaceous glands, arrector pili muscles and both the secretory and excretory portions of eccrine sweat glands. Immunofluorescence screening and laser scanning confocal microscopy showed two subpopulations of dermal TCs; one expressed c-kit/CD117 and the other was positive for CD34. Both subpopulations were also positive for vimentin. The TCs were connected to each other by homocellular junctions, and they formed an interstitial 3D network. We also found TCs adjoined to stem cells in the bulge region of hair follicles. Moreover, TCs established atypical heterocellular junctions with stem cells (clusters of undifferentiated cells). Given the frequency of allergic skin pathologies, we would like to emphasize the finding that close, planar junctions were frequently observed between TCs and mast cells. In conclusion, based on TC distribution and intercellular connections, our results suggested that TCs might be involved in skin homeostasis, skin remodelling, skin regeneration and skin repair.
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影响因子：
3.6
作者：
Hinescu, Mihail E.;Gherghiceanu, Mihaela;Suciu, Laura;Popescu, Laurentiu M.
通讯作者：
Popescu, Laurentiu M.
影响因子：
5.3
作者：
Cantarero Carmona I;Luesma Bartolomé MJ;Junquera Escribano C
通讯作者：
Junquera Escribano C
影响因子：
3.2
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
通讯作者：
Popescu, Laurentziu M.
影响因子：
3.6
作者：
Kruse, Charli;Bodo, Enikoe;Paus, Ralf
通讯作者：
Paus, Ralf
影响因子：
6
作者：
Hachiya, A;Kobayashi, A;Imokawa, G
通讯作者：
Imokawa, G