Genome-Wide Identification and Expression Analysis of the bZIP Transcription Factors in the Mycoparasite Coniothyrium minitans
Genome-Wide Identification and Expression Analysis of the bZIP Transcription Factors in the Mycoparasite Coniothyrium minitans
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真菌寄生虫 Coniothyrium minitans 中 bZIP 转录因子的全基因组鉴定和表达分析
DOI：
10.3390/microorganisms8071045
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发表时间：
2020-07
期刊：
影响因子：
4.5
通讯作者：
Yang Long
中科院分区：
文献类型：
--
作者：
Xu Yuping;Wang Yongchun;Zhao Huizhang;Wu Mingde;Zhang Jing;Chen Weidong;Li Guoqing;Yang Long
碱性亮氨酸拉链（bZIP）蛋白家族是最大且最多样化的转录因子之一，广泛分布于真核生物中。然而，没有关于 Coniothyrium minitans 中 bZIP 基因家族的信息，Coniothyrium minitans 是植物病原体核盘菌的重要生物防治剂。在这项研究中，我们从 C. minitans 基因组中鉴定出了 34 个 bZIP 基因，根据其系统发育关系将其分为 8 组。内含子分析显示，28个CmbZIP基因含有数量不同的内含子，其中15个基因具有内含子插入bZIP结构域的特征。 bZIP结构域的内含子位置高度保守，与识别精氨酸(R)有关，可以视为基因组印记。 CmbZIP 基因响应非生物胁迫的表达分析表明它们可能在非生物胁迫响应中发挥独特的作用。结果表明，22个CmbZIP基因在分生孢子发育后期上调。此外，转录组分析表明 CmbZIP 基因参与真菌寄生的不同阶段。在四个CmbZIPs（CmbZIP07、-09、-13和-16）的缺失突变体中，只有ΔCmbZIP16突变体显着降低了其对氧化应激的耐受性。其他突变体对菌落形态、菌丝生长、分生孢子形成和真菌寄生没有显着影响。综上所述，我们的结果表明 CmbZIP 基因在非生物胁迫反应、分生孢子发育和真菌寄生中发挥重要作用。这些结果提供了CmbZIP基因家族的全面信息，为进一步研究bZIP基因家族的生物学功能和进化史奠定了基础。
The basic leucine zipper (bZIP) proteins family is one of the largest and most diverse transcription factors, widely distributed in eukaryotes. However, no information is available regarding the bZIP gene family in Coniothyrium minitans, an important biocontrol agent of the plant pathogen Sclerotinia sclerotiorum. In this study, we identified 34 bZIP genes from the C. minitans genome, which were classified into 8 groups based on their phylogenetic relationships. Intron analysis showed that 28 CmbZIP genes harbored a variable number of introns, and 15 of them shared a feature that intron inserted into the bZIP domain. The intron position in bZIP domain was highly conserved, which was related to recognize the arginine (R) and could be treated as a genomic imprinting. Expression analysis of the CmbZIP genes in response to abiotic stresses indicated that they might play distinct roles in abiotic stress responses. Results showed that 22 CmbZIP genes were upregulated during the later stage of conidial development. Furthermore, transcriptome analysis indicated that CmbZIP genes are involved in different stages of mycoparasitism. Among deletion mutants of four CmbZIPs (CmbZIP07, -09, -13, and -16), only ΔCmbZIP16 mutants significantly reduced its tolerance to the oxidative stress. The other mutants exhibited no significant effects on colony morphology, mycelial growth, conidiation, and mycoparasitism. Taken together, our results suggested that CmbZIP genes play important roles in the abiotic stress responses, conidial development, and mycoparasitism. These results provide comprehensive information of the CmbZIP gene family and lay the foundation for further research on the bZIP gene family regarding their biological functions and evolutionary history.
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影响因子：
3.9
作者：
Huizhang Zhao;Ting Zhou;Jiatao Xie;Jiasen Cheng;Tao Chen;D. Jiāng;Yanping Fu
通讯作者：
Huizhang Zhao;Ting Zhou;Jiatao Xie;Jiasen Cheng;Tao Chen;D. Jiāng;Yanping Fu
影响因子：
11.6
作者：
Lopez-Berges, Manuel S.;Rispail, Nicolas;Di Pietro, Antonio
通讯作者：
Di Pietro, Antonio
影响因子：
78.5
作者：
P. Lopez-Bergami;E. Lau;Z. Ronai
通讯作者：
P. Lopez-Bergami;E. Lau;Z. Ronai
DOI：
10.1007/bf02423445
发表时间：
1995-09-20
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
KUSANO, T;BERBERICH, T;SUGAWARA, K
通讯作者：
SUGAWARA, K
影响因子：
10.5
作者：
CALLIS, J;FROMM, M;WALBOT, V
通讯作者：
WALBOT, V