The effect of nutritional status on the synthesis ability, protein content and gene expression of mandibular glands in honey bee (Apis mellifera) workers
The effect of nutritional status on the synthesis ability, protein content and gene expression of mandibular glands in honey bee (Apis mellifera) workers
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营养状况对工蜂下颌腺合成能力、蛋白质含量及基因表达的影响
DOI：
10.1080/00218839.2022.2080951
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发表时间：
2022-06
影响因子：
1.9
通讯作者：
Fuliang Hu
中科院分区：
文献类型：
--
作者：
Guozhi Zhang;Yanan Chen;Yuqi Wu;Shuai Wang;Huoqing Zheng;Fuliang Hu
蜜蜂工蜂的下颌腺(MGs)是负责多种蜂王酸生物合成的关键外分泌腺，在蜂群内具有重要的功能。然而，我们对MGs的营养需求的了解相当有限。在这里，我们人工操纵笼养蜜蜂工蜂(花粉加糖糖、纯糖糖、豆粕加糖糖、硬脂酸加糖糖或柠檬酸加糖糖)的营养状况。然后通过测定它们对蜂头中10-羟基-2-癸酸(10-HDA)和10-羟基十一酸(10-HDAA)含量的影响来评价工蜂蜂头微球的合成能力，并对蜂头微球的蛋白质含量和基因表达进行分析，以揭示蜂头微球可能的生理变化。结果表明，营养对麦芽糖的酸合成有显著影响，饲喂花粉的棉铃虫体内10-HDA和10-HDAA含量显著高于只喂糖的棉铃虫，而补充蛋白质和有机酸的影响不显著。此外，营养状况对10-HDA/10-HDAA比值也有显著影响。相反，不同的饲粮对蛋鸡的蛋白质含量和基因表达均无明显影响，但添加有机酸能增加脂肪酸β氧化途径中关键基因的表达。综上所述，我们的研究揭示了蜜蜂的营养状况与工蜂MGs的功能密切相关。营养缺乏对工人MGs的合成能力有显著的负面影响，但对MGs的生理没有影响。
The mandibular glands (MGs) of honey bee workers are key exocrine glands responsible for the biosynthesis of multiple royal jelly acids, which have important functions within the colony. However, our knowledge about the nutrition requirement of MGs is quite limited. Here, we artificially manipulated the nutritional status of caged honey bee workers (pollen and sugar candy, sugar candy only, soybean meal and sugar candy, stearic acid supplemented sugar candy or citric acid supplemented sugar candy). Then we measured their impact on the 10-hydroxy-2-decanoic acid (10-HDA) and 10-hydroxydecanoic acid (10-HDAA) content in honey bee heads to evaluate the synthesis ability of workers’ MGs, and analyzed the protein content of MGs as well as gene expression in the MGs to reveal the possible physiological changes. Our result showed that nutrition strongly impacted the MGs acid synthesis, the 10-HDA and 10-HDAA contents were significantly higher in workers fed with pollen than with sugar candy only, while an additional supply of protein and organic acid showed no significant contribution. In addition, the 10-HDA/10-HDAA ratio was also significantly affected by the nutrition status. On the contrary, different diets showed no influence on the protein content or gene expressions of MGs, except that organic acid supplements could increase the expression of key genes in fatty acid β-oxidation pathways. Taken together, our study revealed that honey bee nutritional status is closely related to the function of worker MGs. The nutritional deficiency has a significant negative impact on the synthesis ability of worker MGs, but showed no effect on the physiology of MGs.
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影响因子：
1.9
作者：
G. DeGrandi-Hoffman;G. Wardell;Fabiana Ahumada-Segura;T. Rinderer;R. Danka;J. Pettis
通讯作者：
G. DeGrandi-Hoffman;G. Wardell;Fabiana Ahumada-Segura;T. Rinderer;R. Danka;J. Pettis
影响因子：
4.6
作者：
Wu Y;Zheng H;Corona M;Pirk C;Meng F;Zheng Y;Hu F
通讯作者：
Hu F
影响因子：
2.2
作者：
Mattila, Heather R.;Otis, Gard W.
通讯作者：
Otis, Gard W.
影响因子：
2.4
作者：
Diniz Frias, Bruna Estefania;Barbosa, Cosme Damiao;Lourenco, Anete Pedro
通讯作者：
Lourenco, Anete Pedro
DOI：
10.1016/j.jchromb.2009.09.016
发表时间：
2009-11-01
影响因子：
3
作者：
Isidorov, V. A.;Czyzewska, U.;Bakier, S.
通讯作者：
Bakier, S.