Detection of respiratory viruses in gargle specimens of healthy children.
Detection of respiratory viruses in gargle specimens of healthy children.
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DOI：
10.1016/j.jcv.2015.01.006
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Kase T
中科院分区：
文献类型：
--
作者：
Morikawa S;Hiroi S;Kase T
从儿童身上采集漱口液样本并检测呼吸道病毒。在45次（45/200; 22.5%）发作中，一些呼吸道病毒检测无症状。在无症状条件下，检出的病毒主要为RV和EV/RV未分型。PIV、RSV和hCoV OC 43仅在出现临床症状时检出。无症状感染可能在病毒循环中发挥重要作用。呼吸道病毒感染是儿童最常见和最重要的疾病之一。聚合酶链反应（PCR）检测常用于检测样本中的病毒，但由于其灵敏度高，PCR阳性的临床意义难以解释，可能反映了既往、即将发生或活动性的无症状感染。虽然在有症状的儿童样本中检测到单一呼吸道病毒，但也可以同时检测到其他呼吸道病毒。然而，这些发现对症状的临床重要性尚不清楚。调查无任何急性呼吸道疾病和/或发热症状的儿童中呼吸道病毒的流行情况。从2013年第25周到2014年第26周，每周一次从儿童中采集漱口液样本，并对这些样本进行实时PCR检测呼吸道病毒。在每个采样日，我们都询问了父母他们孩子的健康状况。在所收集的286个样本中，有200个来自无症状儿童。在无症状的情况下，在45次发作中观察到人双埃柯病毒、腺病毒、肠道病毒、鼻病毒、冠状病毒229 E和HKU 1。在有症状儿童的样本中，除上述病毒外，还检测到副流感病毒、呼吸道合胞病毒和冠状病毒OC 43。在儿童的标本中检测到不同种类的各种病毒，无论其健康状况如何。可以推测，宿主因素如免疫系统的功能影响感染的临床结果。然而，这需要进一步研究。
Gargle samples were collected from children and tested respiratory viruses. In 45(45/200; 22.5%) episodes, some respiratory viruses detected without symptoms. Under asymptomatic conditions, detected viruses were mainly RVs and EV/RV untyped. PIVs, RSV and hCoV OC43 were detected only when clinical symptom was seen. Asymptomatic infections may play an important role in the viral circulation. Respiratory tract viral infection is one of the most common and important diseases in children. Polymerase chain reaction (PCR) tests are often used to detect viruses in samples, it is difficult to interpret the clinical significance of PCR positivity, which may reflect a past, imminent or active asymptomatic infection due to their high sensitivity. Although single respiratory viruses have been detected in samples from children with symptoms, other respiratory viruses can also be detected simultaneously. However, the clinical importance of these findings for the symptoms is not known. To investigate the prevalence of respiratory viruses among children without any symptoms such as acute respiratory illness and/or fever. From week twenty-five 2013 to week twenty-six 2014, gargle samples were collected from children once a week and these samples were subjected to real-time PCR to detect respiratory viruses. On each sampling day, we asked the parents about their children’s health condition. Among the 286 samples collected, 200 were from asymptomatic children. In the asymptomatic condition, human parechovirus, adenovirus, enterovirus, rhinovirus, coronavirus 229E and HKU1 were observed in 45 episodes. In samples from symptomatic children, parainfluenza viruses, respiratory syncytial virus and coronavirus OC43 were detected in addition to those mentioned above. Various viruses of different species were detected in the specimens from the children regardless of their health status. It might be speculated that host factors such as the function of the immune system influence the clinical outcome of the infection. However, this needs to be studied further.
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DOI：
10.1099/vir.0.053686-0
发表时间：
2013-08
期刊：
The Journal of general virology
影响因子：
--
作者：
McIntyre CL;Knowles NJ;Simmonds P
通讯作者：
Simmonds P
影响因子：
4.8
作者：
Debiaggi M;Canducci F;Ceresola ER;Clementi M
通讯作者：
Clementi M
影响因子：
9.4
作者：
Tapparel, Caroline;Cordey, Samuel;Kaiser, Laurent
通讯作者：
Kaiser, Laurent
影响因子：
3.1
作者：
Nakauchi M;Takayama I;Takahashi H;Oba K;Kubo H;Kaida A;Tashiro M;Kageyama T
通讯作者：
Kageyama T
影响因子：
9.4
作者：
Harvala, H.;Robertson, I.;Simmonds, P.
通讯作者：
Simmonds, P.