Proposals for the classification of human rhinovirus species A, B and C into genotypically assigned types.
Proposals for the classification of human rhinovirus species A, B and C into genotypically assigned types.
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DOI：
10.1099/vir.0.053686-0
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Simmonds P
中科院分区：
文献类型：
--
作者：
McIntyre CL;Knowles NJ;Simmonds P
人类鼻病毒 (HRV) 经常引起轻度上呼吸道感染和更严重的疾病表现，例如细支气管炎和哮喘恶化。 HRV 分为小核糖核酸病毒科肠道病毒属的三个物种。 HRV A 种和 B 种含有通过交叉中和测定法鉴定的 75 种和 25 种血清型，尽管使用此类测定法进行常规 HRV 分型受到大量血清型、HRV 诊断中用分子方法替代病毒分离以及细胞培养中 HRV C 种复制不良或不存在的阻碍。为了解决这些问题，我们提出了一种基于 HRV 的遗传相关性的替代基因型分类，类似于肠道病毒所使用的分类。目前分配的 HRV（血清）类型的 384 个完整 VP1 序列之间的核苷酸距离确定了物种 A、B 和 C 的分歧阈值分别为 13%、12% 和 13%，这区分了类型间和类型内的比较。在超过 3800 个 VP4 区域序列的较大数据集中，这些阈值与 10%、9.5% 和 10% 阈值平行。基于 VP1 序列的分配导致对现有类型名称的微小修改（例如将血清型对重新分类，例如 A8/A95 和 A29/A44，作为单一血清型）以及新 HRV 类型 A101-106、B101-103 和 C34-C51 的指定。提出了一种使用 VP1 序列和 VP4 序列比较对类型识别和临时类型分配的限制来分配和编号新 HRV 类型的协议。 HRV 类型的基因型分配和鉴定对于未来研究疾病结果、传播和流行病学中与类型相关的差异具有相当大的价值。
Human rhinoviruses (HRVs) frequently cause mild upper respiratory tract infections and more severe disease manifestations such as bronchiolitis and asthma exacerbations. HRV is classified into three species within the genus Enterovirus of the family Picornaviridae. HRV species A and B contain 75 and 25 serotypes identified by cross-neutralization assays, although the use of such assays for routine HRV typing is hampered by the large number of serotypes, replacement of virus isolation by molecular methods in HRV diagnosis and the poor or absent replication of HRV species C in cell culture. To address these problems, we propose an alternative, genotypic classification of HRV-based genetic relatedness analogous to that used for enteroviruses. Nucleotide distances between 384 complete VP1 sequences of currently assigned HRV (sero)types identified divergence thresholds of 13, 12 and 13 % for species A, B and C, respectively, that divided inter- and intra-type comparisons. These were paralleled by 10, 9.5 and 10 % thresholds in the larger dataset of >3800 VP4 region sequences. Assignments based on VP1 sequences led to minor revisions of existing type designations (such as the reclassification of serotype pairs, e.g. A8/A95 and A29/A44, as single serotypes) and the designation of new HRV types A101–106, B101–103 and C34–C51. A protocol for assignment and numbering of new HRV types using VP1 sequences and the restriction of VP4 sequence comparisons to type identification and provisional type assignments is proposed. Genotypic assignment and identification of HRV types will be of considerable value in the future investigation of type-associated differences in disease outcomes, transmission and epidemiology.
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影响因子：
8.8
作者：
Gaunt, E. R.;Harvala, H.;Simmonds, P.
通讯作者：
Simmonds, P.
影响因子：
5.4
作者：
Ledford, RM;Patel, NR;Pevear, DC
通讯作者：
Pevear, DC
影响因子：
11.8
作者：
Khetsuriani N;Lu X;Teague WG;Kazerouni N;Anderson LJ;Erdman DD
通讯作者：
Erdman DD
影响因子：
12.7
作者：
Harvala, H.;McIntyre, C. L.;Simmonds, P.
通讯作者：
Simmonds, P.
影响因子：
3.1
作者：
COONEY, MK;FOX, JP;KENNY, GE
通讯作者：
KENNY, GE