Porcine oocyte maturation in vitro: role of cAMP and oocyte-secreted factors - A practical approach.
Porcine oocyte maturation in vitro: role of cAMP and oocyte-secreted factors - A practical approach.
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DOI：
10.1262/jrd.2016-016
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发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Kikuchi K
中科院分区：
文献类型：
--
作者：
Appeltant R;Somfai T;Maes D;VAN Soom A;Kikuchi K
猪体外受精（IVF）过程中，多精受精或一个以上精子的穿透仍然是一个问题。猪受精卵经体外培养后，囊胚发育率低，且其质量低于体内囊胚。目前尚不清楚卵母细胞的胞质成熟是否在目前的体外成熟（IVM）程序中得到充分维持。卵母细胞和卵丘细胞在体外成熟过程中复杂的相互作用是这一过程的关键因素。通过关注这种双向通讯，有可能在一定程度上控制IVM期间卵丘扩展以及核和细胞质成熟的协调。因此，本文综述了卵母细胞和卵丘细胞之间的调控机制，以进一步发展新的体外胚胎生产（IVP）程序，从而减少多精受精和提高卵母细胞的发育潜力。具体而言，我们集中在成熟调控和卵母细胞分泌因子（OSFs）的卵母细胞和卵丘细胞之间的双向调节回路中的cAMP的参与。我们的研究表明，维持高cAMP水平的卵母细胞在IVM的前半期持续改善卵母细胞的成熟，导致IVF和卵丘基质解体后的反应增强。最近的研究表明，在IVM过程中添加OSFs可以增强小卵泡来源的卵母细胞的发育能力，这是由表皮生长因子（EGF）通过发育EGF受体信号刺激的。
Polyspermy or the penetration of more than one sperm cell remains a problem during porcine in vitro fertilization (IVF). After in vitro culture of porcine zygotes, only a low percentage of blastocysts develop and their quality is inferior to that of in vivo derived blastocysts. It is unknown whether the cytoplasmic maturation of the oocyte is sufficiently sustained in current in vitro maturation (IVM) procedures. The complex interplay between oocyte and cumulus cells during IVM is a key factor in this process. By focusing on this bidirectional communication, it is possible to control the coordination of cumulus expansion, and nuclear and cytoplasmic maturation during IVM to some extent. Therefore, this review focuses on the regulatory mechanisms between oocytes and cumulus cells to further the development of new in vitro embryo production (IVP) procedures, resulting in less polyspermy and improved oocyte developmental potential. Specifically, we focused on the involvement of cAMP in maturation regulation and function of oocyte-secreted factors (OSFs) in the bidirectional regulatory loop between oocyte and cumulus cells. Our studies suggest that maintaining high cAMP levels in the oocyte during the first half of IVM sustained improved oocyte maturation, resulting in an enhanced response after IVF and cumulus matrix disassembly. Recent research indicated that the addition of OSFs during IVM enhanced the developmental competence of small follicle-derived oocytes, which was stimulated by epidermal growth factor (EGF) via developing EGF-receptor signaling.
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影响因子：
2.8
作者：
Appeltant, R.;Somfai, T.;Van Soom, A.
通讯作者：
Van Soom, A.
影响因子：
2.2
作者：
Day, BN
通讯作者：
Day, BN
影响因子：
3.6
作者：
COSKUN, S;UZUMCU, M;ALAK, BM
通讯作者：
ALAK, BM
影响因子：
3.4
作者：
Bijttebier, Jo;Tilleman, Kelly;Maes, Dominiek
通讯作者：
Maes, Dominiek
影响因子：
2
作者：
Appeltant, Ruth;Somfai, Tamas;Van Soom, Ann
通讯作者：
Van Soom, Ann