An odorant receptor from Anopheles sinensis in China is sensitive to oviposition attractants.
An odorant receptor from Anopheles sinensis in China is sensitive to oviposition attractants.
复制标题
中国中华按蚊的气味受体对产卵引诱剂敏感。
DOI：
10.1186/s12936-018-2501-4
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发表时间：
2018-10-05
期刊：
影响因子：
3
通讯作者：
Gong M
中科院分区：
文献类型：
--
作者：
Liu H;Liu L;Cheng P;Huang X;Gong M
中华按蚊是中国疟疾传播的重要媒介。与嗅觉相关的行为，尤其是寻找产卵地点的行为，为阻断疾病传播过程提供了机会。
这是首次对中华按蚊中AsinOrco和AsinOR10进行鉴定和特性描述的报告。AsinOrco和AsinOR10与冈比亚按蚊中的相关序列分别具有97.49%和90.37%的氨基酸序列同一性。一项功能分析表明，共表达AsinOrco和AsinOR10的HEK293细胞对3 - 甲基吲哚高度敏感，但与二甲基亚砜（DMSO）相比，对其他测试气味物质的反应没有显著差异。
AsinOrco被鉴定为Orco直向同源物亚家族的一个新成员。AsinOR10似乎是OR2 - 10亚家族的成员，直接参与产卵地点的识别。这一发现将有助于阐明中华按蚊嗅觉信号传导的分子机制，并为环境友好型害虫防治提供更多的分子靶点。
本文的网络版（10.1186/s12936 - 2018 - 2501 - 4）包含补充材料，授权用户可获取。
Anopheles sinensis is an important vector for the spread of malaria in China. Olfactory-related behaviours, particularly oviposition site seeking, offer opportunities for disrupting the disease-transmission process. This is the first report of the identification and characterization of AsinOrco and AsinOR10 in An. sinensis. AsinOrco and AsinOR10 share 97.49% and 90.37% amino acid sequence identity, respectively, with related sequences in Anopheles gambiae. A functional analysis demonstrated that AsinOrco- and AsinOR10-coexpressing HEK293 cells were highly sensitive to 3-methylindole, but showed no significant differences in response to other test odorants when compared to DMSO. AsinOrco was characterized as a new member of the Orco ortholog subfamily. AsinOR10, which appears to be a member of the OR2-10 subfamily, is directly involved in identification of oviposition sites. This finding will help to elucidate the molecular mechanisms underlying olfactory signaling in An. sinensis and provide many more molecular targets for eco-friendly pest control. The online version of this article (10.1186/s12936-018-2501-4) contains supplementary material, which is available to authorized users.
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影响因子：
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