Molecular and functional characterization of the odorant receptor2 (OR2) in the tiger mosquito Aedes albopictus.
Molecular and functional characterization of the odorant receptor2 (OR2) in the tiger mosquito Aedes albopictus.
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DOI：
10.1371/journal.pone.0036538
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Digilio FA
中科院分区：
文献类型：
--
作者：
Scialò F;Hansson BS;Giordano E;Polito CL;Digilio FA
在蚊子中，嗅觉系统在许多类型的行为中起着至关重要的作用，包括花蜜喂养，宿主偏好选择和产卵。白纹伊蚊，又称虎蚊，是一种嗜食性物种，近年来由于其强大的生态可塑性，在全球范围内广泛传播。虽然长期以来一直被认为只是病毒的第二媒介，但其潜在的媒介能力可能对公众健康构成威胁。基于对蚊子嗅觉系统的更好理解可能有助于开发干扰其行为的控制方法的想法，我们进行了一项旨在表征A.白纹伊蚊气味受体在这里，我们报告的鉴定，克隆和功能特性的AalOR 2的直系同源物，代表的第一个候选成员的气味受体（OR）家族的蛋白质从A。白纹伊蚊AalOR 2在幼虫头部和成虫触角中表达。我们的数据表明A.白纹伊蚊OR 2（AalOR 2）与迄今为止表征的其他蚊子OR 2直系同源物具有高度同一性，证实OR 2是最保守的蚊子OR之一。我们的数据表明，AalOR 2是狭隘的调整吲哚，并抑制（-）-薄荷酮。与此结果一致，这两种化合物对A.白纹伊蚊幼虫，如通过幼虫行为测定所确定的。总之，这项工作导致了虎蚊A中第一个气味受体的克隆和去孤儿化。白纹伊蚊在未来的控制策略中，这种受体可能被用作潜在的分子靶点。
In mosquitoes, the olfactory system plays a crucial role in many types of behavior, including nectar feeding, host preference selection and oviposition. Aedes albopictus, known also as the tiger mosquito, is an anthropophilic species, which in the last few years, due to its strong ecological plasticity, has spread throughout the world. Although long considered only a secondary vector of viruses, the potential of its vector capacity may constitute a threat to public health. Based on the idea that an improved understanding of the olfactory system of mosquitoes may assist in the development of control methods that interfere with their behavior, we have undertaken a study aimed at characterizing the A. albopictus Odorant Receptors. Here we report the identification, cloning and functional characterization of the AalOR2 ortholog, that represents the first candidate member of the odorant receptor (OR) family of proteins from A. albopictus. AalOR2 is expressed in the larval heads and antennae of adults. Our data indicate that A. albopictus OR2 (AalOR2) shares a high degree of identity with other mosquito OR2 orthologs characterized to date, confirming that OR2 is one of the most conserved mosquito ORs. Our data indicate that AalOR2 is narrowly tuned to indole, and inhibited by (-)-menthone. In agreement with this results, these two compounds elicit two opposite effects on the olfactory-based behavior of A. albopictus larvae, as determined through a larval behavioral assay. In summary, this work has led to the cloning and de-orphaning of the first Odorant Receptor in the tiger mosquito A. albopictus. In future control strategies this receptor may be used as a potential molecular target.
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影响因子：
3.7
作者：
Calzolari M;Bonilauri P;Bellini R;Albieri A;Defilippo F;Maioli G;Galletti G;Gelati A;Barbieri I;Tamba M;Lelli D;Carra E;Cordioli P;Angelini P;Dottori M
通讯作者：
Dottori M
影响因子：
9.8
作者：
Benton, Richard;Sachse, Silke;Michnick, Stephen W;Vosshall, Leslie B
通讯作者：
Vosshall, Leslie B
影响因子：
3.8
作者：
Smart, Renee;Kiely, Aidan;Warr, Coral G.
通讯作者：
Warr, Coral G.
影响因子：
16.2
作者：
Dobritsa, AA;van der Goes van Naters, W;Carlson, JR
通讯作者：
Carlson, JR
影响因子：
2.6
作者：
Bohbot, J.;Pitts, R. J.;Zwiebel, L. J.
通讯作者：
Zwiebel, L. J.